Recombinant Human Anti-Jag1 Antibody scFv Fragment (JAG2.b33) (CAT#: PABX-126-S (P))

Figure 1 Anti-JAG1.b70 and anti-JAG2.b33 specifically block signalling induced by their cognate ligands.

a, ELISA measuring antibody binding to purified protein fragments of human (h) and murine (m) canonical Notch ligands.

Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127.

Figure 2 Anti-JAG1.b70 and anti-JAG2.b33 specifically block signalling induced by their cognate ligands.

Side-view of anti-JAG1 Fab bound to the human JAG1 DSL–EGF1 region. JAG1 residues within 4.5Å of the Fab fragment (HC, heavy chain; LC, light chain) paratope are pink or yellow, with yellow designating residues unique to JAG1, relative to JAG2. Inset shows an ionic interaction between Asp204 (hJAG1) and His35 (HC) that anchors the JAG1–Fab interaction.

Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127.

Figure 3 Immunofluorescent staining for the club cell marker CC10 (green) and the ciliated cell marker acetylated-α-tubulin (acet-α-Tub; red) in distal airway epithelium.

Nuclei counterstained with DAPI. c, Quantification of club and ciliated cell numbers as assessed by immunofluorescent staining for CC10 and FOXJ1, respectively (percentages of each cell type over the total number of airway cells counted, n=5; unpaired t-test, **P<0.01, ***P<0.001).

Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127.

Figure 4 JAG blockade reverses goblet cell metaplasia in vivo.

a, Mice were sensitized during a 34-day period after intraperitoneal (i.p.) injection of ovalbumin (OVA) or vehicle (non-sensitized, b) and then challenged with inhaled ovalbumin to induce inflammation and goblet cell metaplasia. D, day. c–f, Alcian blue/periodic acid-Schiff (PAS) staining for mucin from lung sections of sensitized mice treated with control antibody at the first (day 42) (c) or second (day 48) (d) dose of control antibody, or at the second dose of anti-JAG1.b70.

Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127.