specific is this antibody and what is its source?

This is a fully human monoclonal antibody (clone KYK-1.0) derived from a human library and expressed in CHO cells. It binds with specificity to the human killer cell lectin-like receptor subfamily K member 1 (KLRK1), also known as NKG2D.

What is the biological significance of the KLRK1 target?

KLRK1 (NKG2D) is a transmembrane protein expressed on Natural Killer (NK) cells. It binds to ligands on stressed cells, such as MHC class I chain-related proteins, triggering activation of NK and T cells. It is a significant target for research into immune responses against tumors and viruses.

Anti-Human KLRK1 Recombinant Antibody (KYK-1.0)

CAT#: TAB-520MZ

This fully human monoclonal antibody binds specificity to human killer cell lectin-like receptor subfamily K member 1 (KLRK1). It is recommended for the detection of KLRK1 by ELISA.

Published Data Gene Expression

ELISA

Figure 1 is a graph that depicts the absorbance analysis of KYK-1.0 and KYK-2.0 Fab specificity using ELISA.

Shown is the binding of KYK-1.0 and KYK-2.0 Fab to a panel of proteins immobilized on an ELISA 96-well plate at 100 ng/well. TT11 Fab, which was selected from the same naïve human Fab library against tetanus toxoid, served as control. Error bars indicate mean±SEM (n=3).

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68877/1695_B6_3_cr57d8507991e89_selected.jpg

Normal Tissue Figure 2 High expression in spleen.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21896/ihc_selected.jpg

Normal Tissue Figure 3 Low expression in cerebral cortex.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21896/49188_B_8_5_val_selected.jpg

Normal Tissue Figure 4 Lymph node

Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21896/49188_A_7_8.jpg

Normal Tissue Figure 5 Spleen

Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21896/49188_B_7_4.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enriched (U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213809-KLRK1

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Specifications

Host Species
Human

Derivation
Human library

Type
Human antibody

Antibody Isotype
IgG1

Specificity
Human

Clone
KYK-1.0

Applications
ELISA

Related Disease
Cancer, autoimmune disease, or infectious disease

Applications

Application Notes
This antibody has been reported for use in Enzyme-linked Immunosorbent Assay.

Target

Alternative Names
KLRK1; killer cell lectin-like receptor subfamily K, member 1; KLR; CD314; NKG2D; NKG2-D; D12S2489E; NKG2-D type II integral membrane protein; NK cell receptor D; NKG2-D-activating NK receptor

Gene ID
22914

UniProt ID
A0A024RAP8

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Breast cancer biomarkers at key points during disease progression

Fully Human Antibody

Using a fully human monoclonal antibody (KYK-1.0) was a great advantage for our research on NKG2D receptors. It works very well in ELISA applications, binding specifically to KLRK1, and mimics the natural interaction we wanted to study.

Reliable NKG2D Detection

This product was recommended for the detection of KLRK1, and it did not disappoint. We have used it to investigate the role of this lectin-like receptor in NK cell function, and the data generated has been consistent and reproducible.

Q&As

specific is this antibody and what is its source?

A: This is a fully human monoclonal antibody (clone KYK-1.0) derived from a human library and expressed in CHO cells. It binds with specificity to the human killer cell lectin-like receptor subfamily K member 1 (KLRK1), also known as NKG2D.
What is the biological significance of the KLRK1 target?

A: KLRK1 (NKG2D) is a transmembrane protein expressed on Natural Killer (NK) cells. It binds to ligands on stressed cells, such as MHC class I chain-related proteins, triggering activation of NK and T cells. It is a significant target for research into immune responses against tumors and viruses.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-520MZ.pdf

MSDS

MSDS - TAB-520MZ.pdf

COA

Certificate of Analysis Lookup

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