Anti-Human KLRK1 Recombinant Antibody (KYK-2.0)
CAT#: TAB-521MZ
This human monoclonal antibody recognizes human NKG2D, also known as KLRK1 (killer cell lectin-like receptor subfamily K member 1). It is recommended for the detection of KLRK1 by ELISA and FC.
Published Data
Gene Expression
ELISA
Figure 1 is a graph that depicts the absorbance analysis of KYK-1.0 and KYK-2.0 Fab specificity using ELISA.
Shown is the binding of KYK-1.0 and KYK-2.0 Fab to a panel of proteins immobilized on an ELISA 96-well plate at 100 ng/well. TT11 Fab, which was selected from the same naïve human Fab library against tetanus toxoid, served as control. Error bars indicate mean±SEM (n=3).
FC
Figure 2 is a series of flow cytometry analysis plots of the binding of KYK-2.0 IgG1 to human PBMC subpopulations.
Freshly isolated human PBMC were stained with APC-coupled mouse mAb to CD4, CD8, CD16 and with biotinylated KYK-2.0 IgG1 or TT11-IgG1 (negative control) followed by streptavidin-PE (y axes). PE-coupled mouse anti-human NKG2D mAb 149810 was used as positive control.
Cyt
Figure 3 is a graph that depicts cytolytic activity of ex vivo expanded human NK cells by KYK-2.0 IgG1 measured as specific lysis based on 51Cr release.
Human K562 (top) or Daudi cells (bottom) were labeled with 51Cr and incubated with fresh human NK cells or ex vivo expanded human NK cells at an E:T ratio of 40:1 in the absence or presence of KYK-2.0 IgG1, TT11 IgG1 (negative control), and mouse anti-human NKG2D mAb 149810 (positive control). Error bars indicate mean±SD (n=3); the probability (p) is based on a paired one-tailed t-test.
FuncS
Figure 4 is a series of flow cytometry analysis plots of the degranulation of human NK cells by immobilized KYK-2.0 IgG1.
Freshly isolated human PBMC were stimulated with IL-2 and then incubated with immobilized mAbs. Activation was detected with a mixture of FITC-coupled mouse anti-human CD107a and mouse anti-human CD107b mAbs. NK cells were detected with an APC-coupled mouse anti-human CD56 mAb. CD56+ CD107a/CD107b+ cells are gated (R3). Typical results for one healthy donor are shown. The percentage of degranulated NK cells (CD56+ CD107a/CD107b+) among total NK cells (CD56+) is shown in Table 2 of Example 4 herein for four different healthy donors.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68877/1695_B6_3_cr57d8507991e89_selected.jpg
Normal Tissue
Figure 2 High expression in spleen.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21896/ihc_selected.jpg
Normal Tissue
Figure 3 Low expression in cerebral cortex.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21896/49188_B_8_5_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21896/49188_A_7_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21896/49188_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213809-KLRK1
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Specifications
- Host Species
- Human
- Derivation
- Human library
- Type
- Human antibody
- Specificity
- Human
- Clone
- KYK-2.0
- Applications
- Cyt, ELISA, FC, FuncS
- Related Disease
- Cancer, autoimmune disease, or infectious disease
Applications
- Application Notes
- This antibody has been reported for use in Cytotoxicity, Enzyme-linked Immunosorbent Assay, Flow Cytotoxicityometry, Functional Assay.
Target
- Alternative Names
- KLRK1; killer cell lectin-like receptor subfamily K, member 1; KLR; CD314; NKG2D; NKG2-D; D12S2489E; NKG2-D type II integral membrane protein; NK cell receptor D; NKG2-D-activating NK receptor
- Gene ID
- 22914
- UniProt ID
- A0A024RAP8
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1686z | Mouse Anti-KLRK1 Recombinant Antibody (clone 1H3) | FC, ICC, IF, IHC, IP, FuncS | Mouse IgG1 |
| HPAB-0327-CN | Human Anti-KLRK1 Recombinant Antibody (HPAB-0327-CN) | ELISA, FC, Block | Human IgG4 |
| HPAB-0328-CN | Human Anti-KLRK1 Recombinant Antibody (HPAB-0328-CN) | ELISA, FC, Block | Human IgG4 |
| HPAB-0329-CN | Human Anti-KLRK1 Recombinant Antibody (HPAB-0329-CN) | ELISA, FC, Block | Human IgG4 |
| HPAB-0726-FY | Mouse Anti-KLRK1 Recombinant Antibody (clone AB121) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0002CL | Human Anti-KLRK1 Recombinant Antibody (TAB-0002CL) | ELISA, WB, FC, RIA, Block, IA | Human IgG4 |
| TAB-520MZ | Anti-Human KLRK1 Recombinant Antibody (KYK-1.0) | ELISA | Human antibody |
| TAB-520MZ-S(P) | Anti-Human KLRK1 Recombinant Antibody scFv Fragment (KYK-1.0) | SDS-PAGE, ELISA | Human antibody |
| TAB-520MZ-F(E) | Anti-Human KLRK1 Recombinant Antibody Fab Fragment (KYK-1.0) | SDS-PAGE, ELISA | Human antibody |
| TAB-521MZ-F(E) | Anti-Human KLRK1 Recombinant Antibody Fab Fragment (KYK-2.0) | SDS-PAGE, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1660CQ | Recombinant Mouse Anti-KLRK1 Antibody (5C6) | FC, FuncS, IHC, IP, Neut | IgG2a |
| NEUT-1661CQ | Recombinant Armenian hamster Anti-Klrk1 Antibody (NEUT-1661CQ) | FC, FuncS, Neut | IgG |
| NEUT-1663CQ | Recombinant Rat Anti-Klrk1 Antibody (MI6) | FC, FuncS, Neut | IgG2a, λ |
| NEUT-1667CQ | Recombinant Mouse Anti-KLRK1 Antibody (CBL019) | FC, WB, Neut | IgG1 |
| NEUT-1668CQ | Recombinant Mouse Anti-KLRK1 Antibody (CBL703) | WB, FC, Costim, CyTOF, Neut | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1662CQ | Recombinant Rat Anti-Klrk1 Antibody (VMC202) | FC, BL | IgG1, κ |
| NEUT-1664CQ | Recombinant Hamster Anti-Klrk1 Antibody (VMC203) | BL | IgG |
| NEUT-1665CQ | Recombinant Hamster Anti-Klrk1 Antibody (MAB0840) | BL, FC | IgG2 |
| NEUT-1666CQ | Recombinant Rat Anti-Klrk1 Antibody (CBL347) | BL, CyTOF, FC, WB | IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0620-CN | Llama Anti-KLRK1 Recombinant Antibody (HPAB-0620-CN) | ELISA, FC | Llama VHH |
| HPAB-0621-CN | Llama Anti-KLRK1 Recombinant Antibody (HPAB-0621-CN) | ELISA, FC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0327-CN-S(P) | Human Anti-KLRK1 Recombinant Antibody; scFv Fragment (HPAB-0327-CN-S(P)) | ELISA, FC, Block | Human scFv |
| HPAB-0328-CN-S(P) | Human Anti-KLRK1 Recombinant Antibody; scFv Fragment (HPAB-0328-CN-S(P)) | ELISA, FC, Block | Human scFv |
| HPAB-0329-CN-S(P) | Human Anti-KLRK1 Recombinant Antibody; scFv Fragment (HPAB-0329-CN-S(P)) | ELISA, FC, Block | Human scFv |
| HPAB-0726-FY-F(E) | Mouse Anti-KLRK1 Recombinant Antibody (clone AB121); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0505-WJ-S(P) | Human Anti-KLRK1 Recombinant Antibody; scFv Fragment (HPAB-0505-WJ-S(P)) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0327-CN-F(E) | Human Anti-KLRK1 Recombinant Antibody; Fab Fragment (HPAB-0327-CN-F(E)) | ELISA, FC, Block | Human Fab |
| HPAB-0328-CN-F(E) | Human Anti-KLRK1 Recombinant Antibody; Fab Fragment (HPAB-0328-CN-F(E)) | ELISA, FC, Block | Human Fab |
| HPAB-0329-CN-F(E) | Human Anti-KLRK1 Recombinant Antibody; Fab Fragment (HPAB-0329-CN-F(E)) | ELISA, FC, Block | Human Fab |
| HPAB-0726-FY-S(P) | Mouse Anti-KLRK1 Recombinant Antibody (clone AB121); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0505-WJ-F(E) | Human Anti-KLRK1 Recombinant Antibody; Fab Fragment (HPAB-0505-WJ-F(E)) | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY18 | Human Anti-KLRK1 (clone KYK-2.0) scFv-Fc Chimera | ELISA, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC127 | Recombinant Anti-KLRK1 Vesicular Antibody, EV Displayed (VS-0425-YC127) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3816 | Anti-KLRK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3817 | Anti-Rat KLRK1 Immunohistochemistry Kit | IHC |
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