M31-B01 binds to full length human and mouse C4.4a and can bind to domain S1 of C4.4a but not to domain S2 of C4.4a. The antibody is internalized efficiently.
Figure 1 Provides data on binding of anti C4.4a antibodies of the invention to recombinant human and mouse C4.4a in human IgGl format.
EC50 values were determined by ELISA as described in Example 4. M31-B01 (A) is binding with an EC50 of 0.24 and 0.29 nM to human and murine C4.4a respectively.
Figure 2 Specific internalization of Fluorophor labeledanti-C4.4a antibodies into A549:hC4.4a cells.
(a) provides data on specific internalization of Fluorophor labeledanti-C4.4a antibodies into A549:hC4.4a cells. (b) provides data on non transfected A549 cells as negative control. (A) is M31-B01 (closed squares); (B) is M20-D02 S-A (open squares).
Figure 3 The data on internalization of M31-B01 into A549:hC4.4a cells.
After 5 minutes (c) only a weak staining of the cell surface can be observed. After 90 min (d) intensively coloured endosomes containing the internalized M31-B01 antibody, which is carrying the pH dependent fluorescence dye, are clearly visible and indicate effective internalization.
Figure 4 The epitope M31-B01 and M20-D02 S-A are binding to as determined by western blotting.
(B) was incubated with M31-B01 as detection antibody.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2109 | Hi-Affi™ Recombinant Rabbit Anti-LYPD3 Monoclonal Antibody (DS2109AB) | ELISA | IgG |
MOR-4318 | Hi-Affi™ Recombinant Rabbit Anti-LYPD3 Monoclonal Antibody (SI407DS) | FC | IgG |
MOR-4319 | Hi-Affi™ Recombinant Rabbit Anti-LYPD3 Monoclonal Antibody (SI408DS) | WB, ELISA, ICC/IF, IF, FC | IgG |
MOR-4320 | Hi-Affi™ Recombinant Rabbit Anti-LYPD3 Monoclonal Antibody (SI409DS) | FC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0032CQ | Human Anti-LYPD3 Recombinant Antibody (clone M20-D02S-A) | ELISA, WB, FC, FuncS | Human IgG1, λ |
HPAB-1553LY | Mouse Anti-LYPD3 Recombinant Antibody (HPAB-1553LY) | IHC | Mouse IgG |
HPAB-1554LY | Mouse Anti-LYPD3 Recombinant Antibody (HPAB-1554LY) | IHC | Mouse IgG |
HPAB-1555LY | Mouse Anti-LYPD3 Recombinant Antibody (HPAB-1555LY) | IHC | Mouse IgG |
HPAB-1556LY | Mouse Anti-LYPD3 Recombinant Antibody (HPAB-1556LY) | IHC | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0032CQ-S(P) | Human Anti-LYPD3 Recombinant Antibody (clone M20-D02S-A); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-0033CQ-S(P) | Human Anti-LYPD3 Recombinant Antibody (clone M31-B01); scFv Fragment | ELISA, WB, FC | Human scFv |
HPAB-1553LY-S(P) | Mouse Anti-LYPD3 Recombinant Antibody; scFv Fragment (HPAB-1553LY-S(P)) | IHC | Mouse scfv |
HPAB-1554LY-S(P) | Mouse Anti-LYPD3 Recombinant Antibody; scFv Fragment (HPAB-1554LY-S(P)) | IHC | Mouse scfv |
PABJ-0014-S(P) | Mouse Anti-LYPD3 Recombinant Antibody (clone 11H10); scFv Fragment | ELISA, WB | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1553LY-F(E) | Mouse Anti-LYPD3 Recombinant Antibody; Fab Fragment (HPAB-1553LY-F(E)) | IHC | Mouse Fab |
HPAB-1554LY-F(E) | Mouse Anti-LYPD3 Recombinant Antibody; Fab Fragment (HPAB-1554LY-F(E)) | IHC | Mouse Fab |
HPAB-1555LY-F(E) | Mouse Anti-LYPD3 Recombinant Antibody; Fab Fragment (HPAB-1555LY-F(E)) | IHC | Mouse Fab |
HPAB-1556LY-F(E) | Mouse Anti-LYPD3 Recombinant Antibody; Fab Fragment (HPAB-1556LY-F(E)) | IHC | Mouse Fab |
PABJ-0014-F(E) | Mouse Anti-LYPD3 Recombinant Antibody (clone 11H10); Fab Fragment | ELISA, WB | Mouse Fab |
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