Hi-Affi™ Recombinant Rabbit Anti-MTDH Monoclonal Antibody (TH159DS) (CAT#: MOR-4646)
Recombinant Rabbit anti-MTDH monoclonal antibody for WB, IF, ICC, IHC-P, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/1894_H7_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/1894_H7_3_selected.jpg
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_4_2_selected.jpg
(Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_4_2_selected.jpg
Figure 4 IHC staining of human cerebral corte
(Immunohistochemical staining of human cerebral cortex shows strong positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/ihc_selected.jpg
Figure 5 IHC staining of human smooth muscle
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68205/ihc_selected.jpg
(Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68205/ihc_selected.jpg
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_3.jpg
Figure 7 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_5.jpg
Figure 9 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_6_6.jpg
Figure 10 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_8_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_8_8.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147649-MTDH
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147649-MTDH
Specifications
- Immunogen
- Peptide corresponding to amino acids 561-570 of human Metadherin (C-term)
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human, Mouse
- Clone
- TH159DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Immunohistochemistry (Paraffin); Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 3-5 µg x 10^6 cells; ICC: 1-3 µg/ml; IF: 1:100-1:500; IHC-P: 1:10-1:100; WB: 1-3 µg/ml
Target
- Alternative Names
- Metadherin 2; Lysine-Rich CEACAM1 Co-Isolated Protein; Astrocyte Elevated Gene-1 Protein; Metastasis Adhesion Protein; Astrocyte Elevated Gene 1; 3D3/LYRIC; AEG-1; LYRIC; AEG1; LYRIC/3D3; 3D3
- Gene ID
- 92140
- UniProt ID
- Q86UE4
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-678 | Recombinant Anti-human MTDH Antibody | ELISA, WB, IF, FuncS | IgG |
| HPAB-0497-CN | Mouse Anti-MTDH Recombinant Antibody (clone 1E3) | WB, ELISA, IHC | Mouse IgG1, κ |
| MRO-2030-CN | Rabbit Anti-MTDH Polyclonal Antibody (MRO-2030-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX793 | Mouse Anti-MTDH Recombinant Antibody (clone 2E4-D8-D11) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY969 | Recombinant Mouse Anti-MTDH Antibody (clone 1F3A3) | ELISA, WB, IHC, ICC, FC | Mouse IgG1 |
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