Mouse Anti-MTDH Recombinant Antibody (clone 1E3)
CAT#: HPAB-0497-CN
A monoclonal anti-AEG-1 antibody 1E3 binds to human AEG-1 with high affinity, which is capable of specifically recognizing AEG-1 in cancer cells.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/1894_H7_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_4_2_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human cerebral corte
Immunohistochemical staining of human cerebral cortex shows strong positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68204/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human smooth muscle
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68205/ihc_selected.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10932/158178_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147649-MTDH
❮
❯
Specifications
- Immunogen
- Naïve AEG-1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human MTDH
- Species Reactivity
- Human
- Clone
- 1E3
- Applications
- WB, ELISA, IHC
- Related Disease
- Breast cancer, liver cancer, prostate cancer and melanoma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 1E3"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-678 | Recombinant Anti-human MTDH Antibody | ELISA, WB, IF, FuncS | IgG |
| MRO-2030-CN | Rabbit Anti-MTDH Polyclonal Antibody (MRO-2030-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX793 | Mouse Anti-MTDH Recombinant Antibody (clone 2E4-D8-D11) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-678-F(E) | Recombinant Anti-human MTDH Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-678-F(E) | Recombinant Human Anti-human MTDH Antibody Fab Fragment | WB, IF, FuncS | Fab |
| HPAB-0497-CN-F(E) | Mouse Anti-MTDH Recombinant Antibody (clone 1E3); Fab Fragment | WB, ELISA, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-678-S(P) | Recombinant Anti-human MTDH Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-678-S(P) | Recombinant Human Anti-human MTDH Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| HPAB-0497-CN-S(P) | Mouse Anti-MTDH Recombinant Antibody (clone 1E3); scFv Fragment | WB, ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2316 | Hi-Affi™ Recombinant Rabbit Anti-MTDH Monoclonal Antibody (DS2316AB) | WB, IHC, ICC | IgG |
| MOR-4646 | Hi-Affi™ Recombinant Rabbit Anti-MTDH Monoclonal Antibody (TH159DS) | WB, IF, ICC, IHC-P, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1386 | Anti-MTDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY4559 | Anti-Mouse MTDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY4558 | Anti-Human MTDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY4560 | Anti-Rat MTDH Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC556 | Recombinant Anti-MTDH Vesicular Antibody, EV Displayed (VS-0425-YC556) | ELISA, FC, Cell-uptake |
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