Recombinant Mouse anti-Mouse HSPA8 Monoclonal antibody (24E4)
CAT#: MOB-0939CT
Recombinant Mouse monoclonal antibody to mouse HSPA8, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/987_G4_1_blue_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/802_G12_2_blue_red_green.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_8_4.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109971-HSPA8
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Specifications
- Immunogen
- Mouse spermatogenic cell protein.
- Host Species
- Mouse
- Specificity
- This antibody displays slight cross-reactivity to HSP 70.
- Species Reactivity
- Mouse
- Clone
- 24E4
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunohistochemistry (Frozen sections); Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Ascites
- Format
- Liquid
- Buffer
- PBS with 0.05% sodium azide
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- HSPA8; heat shock protein 8; Hsc70; Hsc71; Hsc73; Hsp73; Hspa10; 2410008N15Rik; heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock 70kD protein 8; heat shock 70 kDa protein 8; heat shock protein cognate 70;
- Gene ID
- 15481
- Protein Refseq
- NP_112442
- Function
- ADP binding; ATP binding; ATPase activity, coupled; ATPase activity, coupled; nucleotide binding; protein binding; receptor binding; unfolded protein binding; unfolded protein binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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