Rabbit Anti-HSPA8 Recombinant Antibody (clone 3G10) (CAT#: ZG-0750J)

Name: Rabbit Anti-HSPA8 Recombinant Antibody (clone 3G10)
SKU: ZG-0750J
Rating: 5 (1 reviews)

This product is a rabbit antibody that recognizes HSPA8.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-HSPA8 Antibody (ZG-0750J) in WB

Figure 1 Rabbit Anti-HSPA8 Antibody (ZG-0750J) in WB

Western Blot
Positive WB detected in: Jurkat whole cell lysate, Colo320 whole cell lysate, U87 whole cell lysate, Raji whole cell lysate
All lanes: HSPA8 antibody at 1.72 μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 71, 54 KDa
Observed band size: 71 KDa

Subcellular Location

Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg

Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/ihc_selected.jpg

Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/ihc_selected.jpg

Figure 4 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/987_G4_1_blue_red_green.jpg

Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/802_G12_2_blue_red_green.jpg

Normal Tissue

Figure 6 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_8_4.jpg

Figure 7 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_7_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109971-HSPA8

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthesized peptide derived from human HSPA8

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human HSPA8

Species Reactivity
Human

Clone
3G10

Applications
ELISA, WB

Conjugate
Uconjugated

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot.
WB:1:500-1:5000

Target

Alternative Names
LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p; heat shock cognate 71 kDa protein; LPS-associated protein 1; N-myristoyltransferase inhibitor protein 71; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; heat shock 70kDa protein 8; heat shock 70kd protein 10; heat shock cognate protein 54; lipopolysaccharide-associated protein 1

Gene ID
3312

UniProt ID
P11142

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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