Mouse Anti-HSPA8 Recombinant Antibody (clone 12B9)
CAT#: MOB-1263z
This product is a mouse antibody that recognizes human HSPA8. It can be used in the following applications: WB, FCM, ICC, IF, IHC, IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/987_G4_1_blue_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/802_G12_2_blue_red_green.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_8_4.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109971-HSPA8
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Specifications
- Immunogen
- Human Heat Shock 70kDa Protein 8
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgM
- Specificity
- Human HSPA8
- Species Reactivity
- Human
- Clone
- 12B9
- Applications
- WB, FC, ICC, IF, IHC, IP
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- HSPA8; Heat Shock 70kd Protein 10; Heat Shock 70kD Protein 8; Lipopolysaccharide-Associated Protein 1; HEL-33; HEL-S-72p; N-Myristoyltransferase Inhibitor Protein 71; NIP71; HSC54; HSPA10; Epididymis Secretory Sperm Binding Protein Li 72p; Constitutive He
- UniProt ID
- P11142
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-0939CT | Recombinant Mouse anti-Mouse HSPA8 Monoclonal antibody (24E4) | ICC, IF, IHC-Fr, IP, WB | |
| MRO-1941-CN | Rabbit Anti-HSPA8 Polyclonal Antibody (MRO-1941-CN) | WB, IF, IHC, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1697 | Rabbit Anti-HSPA8 Recombinant Antibody (clone DS1697AB) | WB, FC, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0525-XY3328 | Anti-HSPA8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3329 | Anti-Mouse HSPA8 Immunohistochemistry Kit | IHC | |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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