Recombinant Rat Anti-NGF Antibody (αD11)
CAT#: PABX-146
Anti-NGF antibody is a Rat antibody of IgG class that binds to an NGF. The rat monoclonal antibody (mAb) has been shown to bind to NGF with nanomolar affinity and with high specificity and to effectively neutralize its in vitro and in vivo biological functions.
Published Data
Tested Data
Gene Expression
Figure 1 IP and WB of transgenic and wild-type mice.
IP and WB of cortex extracts from male(M) and female(F) TgProNGF#72 and wild-type (WT) mice. IP on extracts from cortex(CTX) with anti-NGF aD11 antibody, followed by WB with anti-NGF or anti-proNGF antibody, as described in Tiveron et al.(2013). Are presentative WB probed with anti-proNGF(PAb Alomone),(top)or anti-NGFM20 (Santa Cruz)(bottom) is shown. TgproNGF#72 and wild type mice,male and female,were analyzed.
Malerba, F., Paoletti, F., & Cattaneo, A. (2016). NGF and proNGF reciprocal interference in immunoassays: open questions, criticalities, and ways forward. Frontiers in molecular neuroscience, 9, 63.
Figure 2 EmaxNGF Promega:NGFS tandard curves.
NGF standard curves carried out by usingr at mAb Promega(triangles) and mAb aD11(Cattaneoetal.,1988) (squares) as primary antibody.
Malerba, F., Paoletti, F., & Cattaneo, A. (2016). NGF and proNGF reciprocal interference in immunoassays: open questions, criticalities, and ways forward. Frontiers in molecular neuroscience, 9, 63.
Figure 3 SPR analysis-calibration curve sobtained with proNGF and NG Fover the panel of different antibodies.
Different pro NGF concentrations tested on anti-NGFmAb aD11. ConcentrationsinnM(fromtop): 500,333,222,148,98, 65,33,16,8,4,2,1,0.5.
Malerba, F., Paoletti, F., & Cattaneo, A. (2016). NGF and proNGF reciprocal interference in immunoassays: open questions, criticalities, and ways forward. Frontiers in molecular neuroscience, 9, 63.
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ELISA
Figure 1 Recombinant Rat Anti-NGF Antibody (PABX-146) in ELISA
ELISA analysis of PABX-146 was performed by coating with recombinant human NGF antigen.
SDS-PAGE
Figure 2 Recombinant Rat Anti-NGF Antibody (PABX-146) in SDS-PAGE
SDS-PAGE analysis of PABX-146 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.
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RNA Expression
Figure 1 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134259-NGF
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Rat
- Derivation
- Rat
- Type
- IgG
- Specificity
- Tested positive against native NGF
- Clone
- αD11
- Applications
- Neut (assay dependent)
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
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