Recombinant Rat Anti-NGF Antibody Fab Fragment (αD11)

Name: Recombinant Rat Anti-NGF Antibody Fab Fragment (αD11)
SKU: PABX-146-F (E)
Rating: 5 (1 reviews)

CAT#: PABX-146-F (E)

Anti-NGF antibody is a Rat of Fab class that binds to an NGF. The rat monoclonal antibody (mAb) has been shown to bind to NGF with nanomolar affinity and with high specificity and to effectively neutralize its in vitro and in vivo biological functions.

Published Data Gene Expression

Figure 1 IP and WB of transgenic and wild-type mice.

IP and WB of cortex extracts from male(M) and female(F) TgProNGF#72 and wild-type (WT) mice. IP on extracts from cortex(CTX) with anti-NGF aD11 antibody, followed by WB with anti-NGF or anti-proNGF antibody, as described in Tiveron et al.(2013). Are presentative WB probed with anti-proNGF(PAb Alomone),(top)or anti-NGFM20 (Santa Cruz)(bottom) is shown. TgproNGF#72 and wild type mice,male and female,were analyzed.

Malerba, F., Paoletti, F., & Cattaneo, A. (2016). NGF and proNGF reciprocal interference in immunoassays: open questions, criticalities, and ways forward. Frontiers in molecular neuroscience, 9, 63.

Figure 3 SPR analysis-calibration curve sobtained with proNGF and NG Fover the panel of different antibodies.

Different pro NGF concentrations tested on anti-NGFmAb aD11. ConcentrationsinnM(fromtop): 500,333,222,148,98, 65,33,16,8,4,2,1,0.5.

Malerba, F., Paoletti, F., & Cattaneo, A. (2016). NGF and proNGF reciprocal interference in immunoassays: open questions, criticalities, and ways forward. Frontiers in molecular neuroscience, 9, 63.

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RNA Expression Figure 1 RNA cell line category: Cell line enhanced.

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134259-NGF

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Rat

Derivation
Rat

Type
Fab

Specificity
Tested positive against native NGF

Clone
αD11

Applications
Neut (assay dependent)
FuncS (assay dependent)

Product Property

Purity
>95% by SDS-PAGE and HPLC analysis

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

Application Notes
Assay-depended

Target

Alternative Names
NGF; Beta-NGF; HSAN5; NGFB; PG110; ABT-110

Gene ID
4803

UniProt ID
P01138

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABX-146-F (E).pdf

MSDS

MSDS - PABX-146-F (E).pdf

COA

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