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Anti-NRP2 immunotoxin (scFv)-PE (CAT#: AGTO-G054E)

This immunotoxin was achieved by conjugating/fusing the Anti-NRP2 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating glioblastoma multiforme.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54974/1559_D5_5_selected.jpg
Figure 2 IF staining of human cell line A549
Figure 2 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54974/985_C1_1_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39980/85830_B_7_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39980/85830_A_9_3.jpg
Figure 5 Testis
Figure 5 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39980/85830_A_6_6.jpg
Figure 6 Smooth muscle
Figure 6 Smooth muscle
(Smooth muscle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39980/85830_B_6_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HUVEC TERT2, TIME, U-251 MG)
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HUVEC TERT2, TIME, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000118257-NRP2

Specifications

  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Molecule Class
  • scFv-PE
  • Toxin Description
  • Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
  • Applications
  • This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
  • Related Disease
  • Glioblastoma multiforme

Target

  • Alternative Names
  • NRP2; neuropilin 2; NP2; NPN2; PRO2714; VEGF165R2; neuropilin-2; neuropilin-2b(0); neuropilin-2a(17); neuropilin-2a(22); receptor for VEGF165 and semaphorins class3; vascular endothelial cell growth factor 165 receptor 2; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE80

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Recombinant Antibody

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Fab Fragment Antibody

CAT Product Name Application Type
PFBW-095 Human Anti-NRP2 Recombinant Antibody; Fab Fragment Block Human Fab

scFv Fragment Antibody

CAT Product Name Application Type
PSBW-095 Human Anti-NRP2 Recombinant Antibody; scFv Fragment Block Human scFv

Mouse Antibody

CAT Product Name Application Type
TAB-0152CL Anti-Human NRP2 Recombinant Antibody (ITAC-B1) WB, FC, FuncS, Apop
TAB-0153CL Anti-Human NRP2 Recombinant Antibody (ITAC-B2) WB, FC, FuncS, Apop
TAB-0152CL-S(P) Anti-Human NRP2 Recombinant Antibody scFv Fragment (ITAC-B1) WB, FC, FuncS, Apop
TAB-0152CL-F(E) Anti-Human NRP2 Recombinant Antibody Fab Fragment (ITAC-B1) WB, FC, FuncS, Apop
TAB-0153CL-F(E) Anti-Human NRP2 Recombinant Antibody Fab Fragment (ITAC-B2) WB, FC, FuncS, Apop

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0399MZ Chicken Anti-NRP2 Polyclonal IgY WB Chicken antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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