Anti-NRP2 immunotoxin (scFv)-PE
CAT#: AGTO-G054E
This immunotoxin was achieved by conjugating/fusing the Anti-NRP2 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating glioblastoma multiforme.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54974/1559_D5_5_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54974/985_C1_1_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39980/85830_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39980/85830_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39980/85830_A_6_6.jpg
Normal Tissue
Figure 6 Smooth muscle
Smooth muscle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39980/85830_B_6_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HUVEC TERT2, TIME, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000118257-NRP2
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Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Molecule Class
- scFv-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Glioblastoma multiforme
Target
- Alternative Names
- NRP2; neuropilin 2; NP2; NPN2; PRO2714; VEGF165R2; neuropilin-2; neuropilin-2b(0); neuropilin-2a(17); neuropilin-2a(22); receptor for VEGF165 and semaphorins class3; vascular endothelial cell growth factor 165 receptor 2; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE80
- Gene ID
- 8828
- UniProt ID
- O60462
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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