Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (TH167DS)
CAT#: MOR-4654
Recombinant Rabbit anti-RELA monoclonal antibody for WB, IHC-P, FCM, ChIP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63461/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_7_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18809_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173039-RELA
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Specifications
- Immunogen
- Recombinant protein corresponding to amino acids 375-551 of NF kappaB p65
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human
- Clone
- TH167DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry (Paraffin); Flow Cytometry; Chromatin immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- ChIP: 1-5 µg x 10^6 cells; FC: 3-5 µg/million cells; IHC-P: 1:10-1:50; WB: 1-5 µg/ml
Target
- Alternative Names
- RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; NFKB3; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A; NF-Kappa-B Transcription Factor P65; NF-Kappa-B P65delta3; P65
- Gene ID
- 5970
- UniProt ID
- Q04206
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS13-YC998 | CytoStream™ Rabbit Anti-RELA Recombinant Antibody (VS13-YC998) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0425-FY6 | Rabbit Anti-RelA scFv-Fc Chimera (VS-0425-FY6) | WB, IHC-P, IF, IP | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0525-XY6072 | Anti-RELA Immunohistochemistry Kit | IHC | |
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