Recombinant Anti-human RELA Intrabody [Tat]
CAT#: IAB-B027(T)
Recombinant anti-RELA intrabody in fusion with HIV Tat works within cells against RELA, expressed in E. coli cells. This intrabody against p65 subunit can prevent nuclear factor nB (NF-nB) transcriptional activity in glioma cells andbindinhibit the expression of its target genes involved in the invasion and angiogenesis of human gliomas.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63461/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_7_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18809_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173039-RELA
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Specifications
- Immunogen
- human v-rel avian reticuloendotheliosis viral oncogene homolog A
- Host Species
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Can be useful in applications such as: Flow Cytometry; Immunofluorescence; Functional studies
Product Property
- Purity
- >90% assessed by SDS-PAGE
- Storage
- Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
Target
- Alternative Names
- RELA; v-rel avian reticuloendotheliosis viral oncogene homolog A; p65; NFKB3; transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polype
- Gene ID
- 5970
- UniProt ID
- Q04206
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RELA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B027(A) | Recombinant Anti-human RELA Intrabody [(D-Arg)9] | WB, CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B027(G) | Recombinant Anti-human RELA Intrabody [+36 GFP] | IF, ELISA, FuncS | scFv-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0722MZ | Chicken Anti-Nuclear factor NFκB, p65 subunit (RELA) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3043 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (DS3043AB) | FC, IP, WB | IgG |
| MOR-4654 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (TH167DS) | WB, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0143F | Mouse Anti-RELA Recombinant Antibody (clone 14H2) | WB, IHC-P, IF, IP | Mouse IgG |
| MOB-0144F | Mouse Anti-RELA Recombinant Antibody (clone 5G6) | WB, IHC-P, IF, IP | Mouse IgG |
| VS3-FY1264 | Recombinant Rabbit Anti-RELA Antibody (clone R07-2B6) | WB, ICC, IF | Rabbit IgG |
| VS3-FY1265 | Recombinant Rabbit Anti-RELA Antibody (clone R09-3H2) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-WK2100 | Rabbit Anti-RELA (pS529) Recombinant Antibody (clone R03-6V6) | WB, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY223 | AbPlus™ Anti-RELA Magnetic Beads (CBACN-401) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC998 | CytoStream™ Rabbit Anti-RELA Recombinant Antibody (VS13-YC998) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY6 | Rabbit Anti-RelA scFv-Fc Chimera (VS-0425-FY6) | WB, IHC-P, IF, IP | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6072 | Anti-RELA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6073 | Anti-Mouse RELA Immunohistochemistry Kit | IHC |
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