Recombinant Anti-human RELA Intrabody [+36 GFP]
CAT#: IAB-B027(G)
Recombinant anti-RELA intrabody in fusion with +36GFP works within cells against RELA, expressed in E. coli cells. This intrabody against p65 subunit can prevent nuclear factor nB (NF-nB) transcriptional activity in glioma cells andbindinhibit the expression of its target genes involved in the invasion and angiogenesis of human gliomas.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63461/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_7_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18809_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173039-RELA
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Specifications
- Immunogen
- human v-rel avian reticuloendotheliosis viral oncogene homolog A
- Host Species
- human
- Type
- scFv-(+36GFP)
- Cell Penetrating Peptide
- +36 GFP
GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.
- Species Reactivity
- human
- Applications
- Products can be used for: Immunofluorescence; Enzyme-linked Immunosorbent Assay; Functional studies
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- Store at -20°C. Open under aseptic conditions.
Target
- Alternative Names
- RELA; v-rel avian reticuloendotheliosis viral oncogene homolog A; p65; NFKB3; transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polype
- Gene ID
- 5970
- UniProt ID
- Q04206
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RELA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B027(A) | Recombinant Anti-human RELA Intrabody [(D-Arg)9] | WB, CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B027(T) | Recombinant Anti-human RELA Intrabody [Tat] | FC, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0722MZ | Chicken Anti-Nuclear factor NFκB, p65 subunit (RELA) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3043 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (DS3043AB) | FC, IP, WB | IgG |
| MOR-4654 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (TH167DS) | WB, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX968 | Mouse Anti-RELA Recombinant Antibody (clone 5F4-4E1-5H8) | IHC | Mouse IgG1 |
| VS3-QX969 | Mouse Anti-RELA Recombinant Antibody (clone 6D6-4F5-10H4) | IHC | Mouse IgG1 |
| VS3-QX970 | Mouse Anti-RELA Recombinant Antibody (clone 4A9-B3-D8) | WB | Mouse IgG2a |
| VS3-FY1264 | Recombinant Rabbit Anti-RELA Antibody (clone R07-2B6) | WB, ICC, IF | Rabbit IgG |
| VS3-FY1265 | Recombinant Rabbit Anti-RELA Antibody (clone R09-3H2) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY223 | AbPlus™ Anti-RELA Magnetic Beads (CBACN-401) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC998 | CytoStream™ Rabbit Anti-RELA Recombinant Antibody (VS13-YC998) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY6 | Rabbit Anti-RelA scFv-Fc Chimera (VS-0425-FY6) | WB, IHC-P, IF, IP | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6072 | Anti-RELA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6073 | Anti-Mouse RELA Immunohistochemistry Kit | IHC |
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