Recombinant Anti-human SGK1 Intrabody [(D-Arg)9]
CAT#: IAB-B057(A)
Recombinant anti-SGK1 intrabody linked to (D-Arg)9 works within cells to inhibit the activity of human SGK1, expressed in 293T cells. The anti-Sgk intrabodies recognize the enzymatically active form of Sgk and which, upon binding, inhibit Sgk's ability to transphosphorylate.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51251/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25148/56215_A_2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/49011_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HHSteC, RPTEC TERT1, SK-MEL-30, TIME, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118515-SGK1
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Specifications
- Immunogen
- human serum/glucocorticoid regulated kinase 1
- Host Species
- human
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Products can be used for: Western blot; Neutralization; Functional studies
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Related Signaling Pathways
mTOR Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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|---|---|---|---|
| IAB-B057(G) | Recombinant Anti-human SGK1 Intrabody [+36 GFP] | WB, ICC, Neut, FuncS | scFv-(+36GFP) |
| IAB-B057(T) | Recombinant Anti-human SGK1 Intrabody [Tat] | PCA, Neut, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
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| MOB-1839MZ | Recombinant Mouse Anti-Human SGK1 Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| MRO-1387-CN | Recombinant Rabbit Anti-SGK1 Monoclonal Antibody (CBACN-499) | WB, IHC, IP, FC | Rabbit IgG |
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| VS3-QX1007 | Mouse Anti-SGK1 Recombinant Antibody (clone 6E4-8G5-6D10) | IHC | Mouse IgG1 |
| VS3-CJ113 | Rabbit Anti-SGK1 Recombinant Antibody (VS3-CJ113) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| BRD-0530MZ | Chicken Anti-SGK (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0531MZ | Chicken Anti-SGK (ab2) Polyclonal IgY | WB | Chicken antibody |
| BRD-0761MZ | Chicken Anti-SGK1 Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
| BRD-1049MZ | Chicken Anti-SGK1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3249 | Hi-Affi™ Recombinant Rabbit Anti-SGK1 Monoclonal Antibody (DS3249AB) | WB, FC, IP, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC419 | AbPlus™ Anti-SGK1 Magnetic Beads (VS-0724-YC419) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2059 | Anti-SGK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6541 | Anti-Mouse SGK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6540 | Anti-Human SGK1 Immunohistochemistry Kit | IHC |
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