Recombinant Anti-human SGK1 Intrabody [Tat]
CAT#: IAB-B057(T)
Recombinant anti-SGK1 intrabody in fusion with HIV Tat works within cells to inhibit the activity of human SGK1, expressed in 293T cells. The anti-Sgk intrabodies recognize the enzymatically active form of Sgk and which, upon binding, inhibit Sgk's ability to transphosphorylate.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51251/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25148/56215_A_2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/49011_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HHSteC, RPTEC TERT1, SK-MEL-30, TIME, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118515-SGK1
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Specifications
- Immunogen
- human serum/glucocorticoid regulated kinase 1
- Host Species
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Protein fragment complementation; Neutralization; Functional studies
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Target
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Related Signaling Pathways
mTOR Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B057(A) | Recombinant Anti-human SGK1 Intrabody [(D-Arg)9] | WB, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B057(G) | Recombinant Anti-human SGK1 Intrabody [+36 GFP] | WB, ICC, Neut, FuncS | scFv-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1839MZ | Recombinant Mouse Anti-Human SGK1 Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| MRO-1387-CN | Recombinant Rabbit Anti-SGK1 Monoclonal Antibody (CBACN-499) | WB, IHC, IP, FC | Rabbit IgG |
| MOB-0243F | Mouse Anti-SGK1 Recombinant Antibody (clone 4D12) | IHC-P, IF | Mouse IgG |
| VS3-QX1007 | Mouse Anti-SGK1 Recombinant Antibody (clone 6E4-8G5-6D10) | IHC | Mouse IgG1 |
| VS4-CJ113 | Recombinant Mouse Anti-SGK1 Antibody (VS4-CJ113) | ELISA, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0530MZ | Chicken Anti-SGK (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0531MZ | Chicken Anti-SGK (ab2) Polyclonal IgY | WB | Chicken antibody |
| BRD-0761MZ | Chicken Anti-SGK1 Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
| BRD-1049MZ | Chicken Anti-SGK1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3249 | Hi-Affi™ Recombinant Rabbit Anti-SGK1 Monoclonal Antibody (DS3249AB) | WB, FC, IP, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC419 | AbPlus™ Anti-SGK1 Magnetic Beads (VS-0724-YC419) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2059 | Anti-SGK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6541 | Anti-Mouse SGK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6540 | Anti-Human SGK1 Immunohistochemistry Kit | IHC |
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