Recombinant Anti-human SGK1 Intrabody [Tat] (CAT#: IAB-B057(T))
Recombinant anti-SGK1 intrabody in fusion with HIV Tat works within cells to inhibit the activity of human SGK1, expressed in 293T cells. The anti-Sgk intrabodies recognize the enzymatically active form of Sgk and which, upon binding, inhibit Sgk's ability to transphosphorylate.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51251/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51251/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/if_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25148/56215_A_2_1_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25148/56215_A_2_1_selected.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/49011_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/49011_A_6_6.jpg
Figure 5 RNA cell line category: Cell line enhanced (HHSteC, RPTEC TERT1, SK-MEL-30, TIME, U-266/84)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118515-SGK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118515-SGK1
Specifications
- Immunogen
- human serum/glucocorticoid regulated kinase 1
- Host Species
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Protein fragment complementation; Neutralization; Functional studies
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Target
- Alternative Names
- SGK1; serum/glucocorticoid regulated kinase 1; SGK; serine/threonine-protein kinase Sgk1; Sgk1 variant i3; serine/threonine protein kinase SGK; serum/glucocorticoid-regulated kinase 1
- Gene ID
- 6446
- UniProt ID
- O00141
Related Resources
mTOR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "SGK1"
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B057(A) | Recombinant Anti-human SGK1 Intrabody [(D-Arg)9] | WB, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B057(G) | Recombinant Anti-human SGK1 Intrabody [+36 GFP] | WB, ICC, Neut, FuncS | scFv-(+36GFP) |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1839MZ | Recombinant Mouse Anti-Human SGK1 Antibody | ICC/IF, IHC-P, WB | Mouse antibody |
| MRO-1387-CN | Recombinant Rabbit Anti-SGK1 Monoclonal Antibody (CBACN-499) | WB, IHC, IP, FC | Rabbit IgG |
| MOB-0243F | Mouse Anti-SGK1 Recombinant Antibody (clone 4D12) | IHC-P, IF | Mouse IgG |
| VS3-QX1007 | Mouse Anti-SGK1 Recombinant Antibody (clone 6E4-8G5-6D10) | IHC | Mouse IgG1 |
| VS3-CJ113 | Rabbit Anti-SGK1 Recombinant Antibody (VS3-CJ113) | WB, IHC, IP, FC | Rabbit IgG |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0530MZ | Chicken Anti-SGK (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0531MZ | Chicken Anti-SGK (ab2) Polyclonal IgY | WB | Chicken antibody |
| BRD-0761MZ | Chicken Anti-SGK1 Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
| BRD-1049MZ | Chicken Anti-SGK1 Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3249 | Hi-Affi™ Recombinant Rabbit Anti-SGK1 Monoclonal Antibody (DS3249AB) | WB, FC, IP, IHC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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