Mouse Anti-SIGLEC6 Recombinant Antibody (clone Mab239-90)

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Citations

1. Sen Santara, Sumit, et al. "The NK cell receptor NKp46 recognizes ecto-calreticulin on ER-stressed cells." Nature 616.7956 (2023): 348-356. https://doi.org/10.1038/s41586-023-05912-0
   This research identifies ecto-calreticulin (ecto-CRT) as the endogenous ligand for NKp46, an evolutionarily ancient natural killer (NK) cell receptor. The study demonstrates that NKp46 specifically binds to calreticulin when it translocates from the endoplasmic reticulum to the cell surface during cellular stress conditions, including viral infection, chemotherapy-induced immunogenic cell death, and senescence. Through extensive biochemical, cellular, and in vivo experiments, the researchers show that this interaction activates NK cells to kill stressed cells. The NKp46-CRT interaction occurs through specific residues in the P domain of calreticulin and appears conserved between humans and mice. In mouse models, NKp46 recognition of ecto-CRT significantly suppressed tumor growth and metastasis in an NK cell-dependent manner.
   Creative Biolabs provided anti-human Siglec-6 antibodies (clone Mab239-90) that were used to investigate potential alternative ligands for NKp46. These antibodies helped the researchers assess whether Siglec-6, which was shown to bind NKp46 with higher affinity than ecto-CRT, played a role in NK cell activation. The experiments using these antibodies revealed that despite stronger binding, Siglec-6 did not significantly affect NK cell killing of ZIKV-infected cells, which was crucial for establishing ecto-CRT as the functionally relevant endogenous ligand responsible for NKp46-mediated immunosurveillance.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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