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    Hi-Affi™ Recombinant Rabbit Anti-SMAD1 Monoclonal Antibody (TH222DS) (CAT#: MOR-4708)

    Recombinant Rabbit anti-SMAD1 monoclonal antibody for WB, IF, ICC, IHC-P, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human stomach
    Figure 2 IHC staining of human stomach
    (Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_5_1_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_B_7_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_9_3.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_8_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_6_6.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_9_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Cell line enhanced (HUVEC TERT2)
    Figure 8 RNA cell line category: Cell line enhanced (HUVEC TERT2)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170365-SMAD1

    Specifications

    • Immunogen
    • APeptide corresponding to amino acids 456-465 of Q15797 and Q99717.
    • Host Species
    • Rabbit
    • Type
    • IgG
    • Species Reactivity
    • Human
    • Clone
    • TH222DS
    • Applications
    • Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Immunohistochemistry (Paraffin); Flow Cytometry
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Absolute Mag™ Protein A Agarose
    • Purity
    • > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
    • Format
    • Liquid
    • Buffer
    • PBS
    • Preservative
    • 0.09% sodium azide
    • Storage
    • Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • FC: 0.5-5 µg x 10^6 cells; ICC: 2-4 µg/ml; IF: 2-4 µg/ml; IHC-P: 4-6 µg/ml; WB: 1-2 µg/ml

    Target

    • Alternative Names
    • SMAD Family Member 1; Mothers Against DPP Homolog 1; Mad-Related Protein 1; MADH1; BSP-1; JV4-1; MADR1; BSP1; MAD, Mothers Against Decapentaplegic Homolog 1 (Drosophila); Transforming Growth Factor-Beta Signaling Protein 1; Transforming Growth Factor-Beta-Signaling Protein 1; SMAD, Mothers Against DPP Homolog 1 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 1; SMAD, Mothers Against DPP Homolog 1; TGF-Beta Signaling Protein 1; MAD Homolog 1; HsMAD1; SMAD 1; SMAD1; JV41

    Related Resources

    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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