Anti-Staphylococcal enterotoxin B (SEB) VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Figure 1 Thermal denaturation of Construct 1 monitored by CD, DSC, and fluorescent dye assay.
The high melting point of Construct 1 is ambiguous but above 90°C by multiple techniques.
Figure 2 Binding affinity of mutant constructs.
Surface plasmon resonance data is shown for each sdAb. Protein concentration was 300 nM and the data are offset by 10 units each for clarity of the base line. Construct identity is shown by orange labels. Little to no effect on the on-rate or off-rate is observed.
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