Recombinant Anti-Staphylococcal SEB VHH Single Domain Antibody (PNBL-029) (CAT#: PNBL-029)

Anti-Staphylococcal enterotoxin B (SEB) VHH Single Domain Antibody is a recombinant protein produced in E. coli.

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  • Conjugation:

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  • Purity:

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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Thermal denaturation of Construct 1 monitored by CD, DSC, and fluorescent dye assay.

Figure 1 Thermal denaturation of Construct 1 monitored by CD, DSC, and fluorescent dye assay.

The high melting point of Construct 1 is ambiguous but above 90°C by multiple techniques.

Zabetakis, D., Olson, M. A., Anderson, G. P., Legler, P. M., & Goldman, E. R. (2014). Evaluation of disulfide bond position to enhance the thermal stability of a highly stable single domain antibody. PLoS One, 9(12), e115405.

SPR

Figure 2 Binding affinity of mutant constructs.

Figure 2 Binding affinity of mutant constructs.

Surface plasmon resonance data is shown for each sdAb. Protein concentration was 300 nM and the data are offset by 10 units each for clarity of the base line. Construct identity is shown by orange labels. Little to no effect on the on-rate or off-rate is observed.

Zabetakis, D., Olson, M. A., Anderson, G. P., Legler, P. M., & Goldman, E. R. (2014). Evaluation of disulfide bond position to enhance the thermal stability of a highly stable single domain antibody. PLoS One, 9(12), e115405.


Specifications

  • Immunogen
  • Staphylococcal enterotoxin B
  • Host Species
  • Llama
  • Derivation
  • Llama
  • Type
  • Llama VHH
  • Species Reactivity
  • Staphylococcal
  • Clone
  • PNBL-029
  • Applications
  • Surface plasmon resonance

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody was validated for Surface plasmon resonance. For details, refer to Published Data.

Target

  • Alternative Names
  • SEB; Staphylococcal enterotoxin B; enterotoxin type B

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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