Recombinant Anti-Staphylococcal SEB VHH Single Domain Antibody (PNBL-029) (CAT#: PNBL-029)
Anti-Staphylococcal enterotoxin B (SEB) VHH Single Domain Antibody is a recombinant protein produced in E. coli.
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Figure 1 Thermal denaturation of Construct 1 monitored by CD, DSC, and fluorescent dye assay.
The high melting point of Construct 1 is ambiguous but above 90°C by multiple techniques.
Zabetakis, D., Olson, M. A., Anderson, G. P., Legler, P. M., & Goldman, E. R. (2014). Evaluation of disulfide bond position to enhance the thermal stability of a highly stable single domain antibody. PLoS One, 9(12), e115405.
SPR
Figure 2 Binding affinity of mutant constructs.
Surface plasmon resonance data is shown for each sdAb. Protein concentration was 300 nM and the data are offset by 10 units each for clarity of the base line. Construct identity is shown by orange labels. Little to no effect on the on-rate or off-rate is observed.
Zabetakis, D., Olson, M. A., Anderson, G. P., Legler, P. M., & Goldman, E. R. (2014). Evaluation of disulfide bond position to enhance the thermal stability of a highly stable single domain antibody. PLoS One, 9(12), e115405.
Specifications
- Immunogen
- Staphylococcal enterotoxin B
- Host Species
- Llama
- Derivation
- Llama
- Type
- Llama VHH
- Species Reactivity
- Staphylococcal
- Clone
- PNBL-029
- Applications
- Surface plasmon resonance
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for Surface plasmon resonance. For details, refer to Published Data.
Target
- Alternative Names
- SEB; Staphylococcal enterotoxin B; enterotoxin type B
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
ScFv Antibody Products
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IgG Antibody Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-895-F(E) | Recombinant Anti-SEB Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
| MHH-895-F(E) | Recombinant Human Anti-SEB Antibody Fab Fragment | WB, IP, FuncS | Fab |
| PFBL-336 | Recombinant Human Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (3E2) | WB, ELISA, Neut, FuncS | Fab |
| PFBL-337 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (20B1) | Neut, FuncS | Fab |
| HPAB-0090-FY-F(E) | Recombinant Mouse Anti-SEB Antibody Fab Fragment (FMU-SEB-No.2) | E |
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