Recombinant Anti-Staphylococcal SEB VHH Single Domain Antibody (A3) (CAT#: PNBL-029)

Anti-Staphylococcal enterotoxin B (SEB) VHH Single Domain Antibody is a recombinant protein produced in E. coli.

  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Thermal denaturation of Construct 1 monitored by CD, DSC, and fluorescent dye assay.

The high melting point of Construct 1 is ambiguous but above 90°C by multiple techniques.

Zabetakis, D., Olson, M. A., Anderson, G. P., Legler, P. M., & Goldman, E. R. (2014). Evaluation of disulfide bond position to enhance the thermal stability of a highly stable single domain antibody. PLoS One, 9(12), e115405.

SPR

Figure 2 Binding affinity of mutant constructs.

Surface plasmon resonance data is shown for each sdAb. Protein concentration was 300 nM and the data are offset by 10 units each for clarity of the base line. Construct identity is shown by orange labels. Little to no effect on the on-rate or off-rate is observed.

Zabetakis, D., Olson, M. A., Anderson, G. P., Legler, P. M., & Goldman, E. R. (2014). Evaluation of disulfide bond position to enhance the thermal stability of a highly stable single domain antibody. PLoS One, 9(12), e115405.


Specifications

  • Immunogen
  • Staphylococcal enterotoxin B
  • Host Species
  • Llama
  • Derivation
  • Llama
  • Type
  • Llama VHH
  • Species Reactivity
  • Staphylococcal
  • Clone
  • A3
  • Applications
  • Surface plasmon resonance

Applications

  • Application Notes
  • The antibody was validated for Surface plasmon resonance. For details, refer to Published Data.

Target

  • Alternative Names
  • SEB; Staphylococcal enterotoxin B; enterotoxin type B

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "SEB"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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