Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) (CAT#: TAB-168CQ)

Figure 1 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in SDS-PAGE

SDS-PAGE analysis of TAB-168CQ in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in SEC-HPLC

The purity of TAB-168CQ was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in WB

Western blot analysis of TAB-168CQ was performed by loading Human TREM-2 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-168CQ and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)

Figure 4 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in DB

Dot Blot analysis of TAB-168CQ was performed by coating with Human TREM-2 Protein (His Tag).

TAB-168CQ incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

Figure 5 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in ELISA

ELISA analysis of TAB-168CQ was performed by coating with Human TREM-2 Protein (His Tag). Then blocked with BSA, and incubated with TAB-168CQ. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.