This product is a recombinant mouse antibody that recognizes TREM2. The antibody was purified by affinity chromatography. It can be used for WB, ELISA, FC, IHC, IF, IP and other applications.
Figure 1 Chimeric (Mouse/Human) Anti-TREM2 Recombinant Antibody (HPAB-0155-CN) in SDS-PAGE
SDS-PAGE analysis of HPAB-0155-CN in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 25 kDa and 50 kDa, respectively.
Figure 2 Chimeric (Mouse/Human) Anti-TREM2 Recombinant Antibody (HPAB-0155-CN) in SEC-HPLC
The purity of HPAB-0155-CN was 97.2042% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Chimeric (Mouse/Human) Anti-TREM2 Recombinant Antibody (HPAB-0155-CN) in WB
Western blot analysis of HPAB-0155-CN was performed by loading human TREM2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0155-CN and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 4 Chimeric (Mouse/Human) Anti-TREM2 Recombinant Antibody (HPAB-0155-CN) in DB
Dot Blot analysis of HPAB-0155-CN was performed by coating with human TREM2 protein (His tag).
HPAB-0155-CN incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
Figure 5 Chimeric (Mouse/Human) Anti-TREM2 Recombinant Antibody (HPAB-0155-CN) in ELISA
ELISA analysis of HPAB-0155-CN was performed by coating with human TREM2 protein (His tag). Then blocked with BSA and incubated with HPAB-0155-CN. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-168CQ | Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) | ELISA, FC, WB, FuncS | Human IgG |
TAB-169CQ | Human Anti-TREM2 Recombinant Antibody (TAB-169CQ) | ELISA, FC, WB, FuncS | Human IgG |
TAB-168CQ-S(P) | Human Anti-TREM2 Recombinant Antibody; scFv Fragment (TAB-168CQ-S(P)) | ELISA, FC, WB, FuncS | Human scFv |
TAB-168CQ-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (TAB-168CQ-F(E)) | ELISA, FC, WB, FuncS | Human Fab |
TAB-169CQ-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (TAB-169CQ-F(E)) | ELISA, FC, WB, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0152-CN-F(E) | Human Anti-TREM2 Recombinant Antibody (clone Pil.02.002); Fab Fragment | ELISA, FC | Human Fab |
HPAB-0153-CN-F(E) | Human Anti-TREM2 Recombinant Antibody (clone Pil.02.005); Fab Fragment | ELISA, FC | Human Fab |
HPAB-0154-CN-F(E) | Mouse Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-0154-CN-F(E)) | WB, ELISA, FC, IHC, IF, IP | Mouse Fab |
HPAB-0155-CN-F(E) | Mouse Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-0155-CN-F(E)) | WB, ELISA, FC, IHC, IF, IP | Mouse Fab |
HPAB-0156-CN-F(E) | Mouse Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-0156-CN-F(E)) | WB, ELISA, FC, IHC, IF, IP | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-3341LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3341LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
HPAB-3342LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3342LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
HPAB-3343LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3343LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
HPAB-3344LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3344LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
HPAB-3345LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3345LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-3372LY-S(P) | Human Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-3372LY-S(P)) | FC, WB, IP, ELISA, IHC | Humanized scfv |
HPAB-3373LY-S(P) | Human Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-3373LY-S(P)) | FC, WB, IP, ELISA, IHC | Humanized scfv |
HPAB-3374LY-S(P) | Human Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-3374LY-S(P)) | FC, WB, IP, ELISA, IHC | Humanized scfv |
HPAB-3375LY-S(P) | Human Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-3375LY-S(P)) | FC, WB, IP, ELISA, IHC | Humanized scfv |
HPAB-3376LY-S(P) | Human Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-3376LY-S(P)) | FC, WB, IP, ELISA, IHC | Humanized scfv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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