Human Anti-VEGFR2 Recombinant Antibody (clone 1121B)
CAT#: PABL-743
Recombinant Human Antibody (1121B) is capable of binding to VEGFR2, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-VEGFR2 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-VEGFR2 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The anti-VEGF receptor 2 antibody is a promising antiangiogenic drug being tested for treatment of breast and gastric cancer.
Published Data
Tested Data
Figure 1 The pro-invasive effect of HUVECs or bone marrow cells on cancer cells.
(A, B) Invasion chamber assay was used to evaluate the invasion of KYSE270 cells attracted by HUVECs in the presence or absence of IMC-18F1 and IMC-1121B. (C, D) The ability of sorted VEGFR1 + and VEGFR2 + bone marrow cells to attract invading KYSE270 cancer cells was determined. Bars, SD; **, P < 0.01, ***, P < 0.001 compared with the cells treated with VEGF and isotype IgG.
Xu, W. , Li, B. , Lam, A. , Tsao, G. , Law, S. , & Chan, K. , et al. (2015). Targeting vegfr-1 and vegfr2-expressing non-tumor cells is essential for esophageal cancer therapy. Oncotarget, 6(3), 1790-805.
Figure 2 KDR-Blocking ELISAs.
(A) 1121B Fab blocking the binding of KDR d1–3-Fc, d1–7-Fc, or d1–7-AP to VEGF-165. Points and error bars represent mean ± SEM of duplicate or triplicate measurements. Curves are nonlinear fits to a single-site inhibition equation, with fixed KD and concentration for the KDR-VEGF interaction as described in Experimental Procedures.
Franklin, M. , Navarro, E. , Wang, Y. , Patel, S. , Singh, P. , & Zhang, Y. , et al. (2011). The structural basis for the function of two anti-vegf receptor 2 antibodies. Structure, 19(8), 1097-1107.
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SDS-PAGE
Figure 1 Human Anti-VEGFR2 Recombinant Antibody (PABL-743) in SDS-PAGE
Lane 1: Reducing (SDS-PAGE)
Lane 2: Non-Reducing (SDS-PAGE)
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Specifications
- Immunogen
- Human kinase insert domain receptor
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human VEGFR2
- Species Reactivity
- Human
- Clone
- 1121B
- Applications
- WB, IF, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The VEGFR2 antibody has been reported in applications of Invasion, ELISA.
ELISA Binding ELISAs were performed by coating KDR d1-3-Fc wild-type, and the mutants derived from it, on the surface of Immulon 2 HB plates (Thermo Scientific), blocking, washing, and then applying serial dilutions of the appropriate binding partner (VEGF-165, 1121B Fab, or 6.64 Fab) and incubating at room temperature for 1 hr.
Target
- Alternative Names
- KDR; kinase insert domain receptor; FLK1; CD309; VEGFR; VEGFR2; vascular endothelial growth factor receptor 2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor; kinase insert domain receptor (a type III receptor tyrosine kinase)
- Gene ID
- 3791
- UniProt ID
- A0A024RD88
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-736 | Human Anti-VEGFR2 Recombinant Antibody (clone 1121B); scFv Fragment | WB, IF, FuncS | Human scFv |
| HPAB-0041LY-S(P) | Human Anti-VEGFR2 Recombinant Antibody; scFv Fragment (HPAB-0041LY-S(P)) | WB | Human scfv |
| HPAB-2710LY-S(P) | Mouse Anti-VEGFR2 Recombinant Antibody; scFv Fragment (HPAB-2710LY-S(P)) | ELISA, Inhib | Mouse scfv |
| HPAB-2711LY-S(P) | Mouse Anti-VEGFR2 Recombinant Antibody; scFv Fragment (HPAB-2711LY-S(P)) | ELISA, Inhib | Mouse scfv |
| HPAB-2712LY-S(P) | Mouse Anti-VEGFR2 Recombinant Antibody; scFv Fragment (HPAB-2712LY-S(P)) | ELISA, Inhib | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0021CL | Human Anti-VEGFR2 Recombinant Antibody (TAB-0021CL) | ELISA, Block | Human IgG1 |
| TAB-320CQ-F(E) | Human Anti-VEGFR2 Recombinant Antibody; Fab Fragment (TAB-320CQ-F(E)) | ELISA, WB | Human Fab |
| TAB-321CQ-F(E) | Human Anti-VEGFR2 Recombinant Antibody; Fab Fragment (TAB-321CQ-F(E)) | ELISA, WB | Human Fab |
| TAB-323CQ-F(E) | Human Anti-VEGFR2 Recombinant Antibody; Fab Fragment (TAB-323CQ-F(E)) | ELISA, WB | Human Fab |
| TAB-324CQ-F(E) | Human Anti-VEGFR2 Recombinant Antibody; Fab Fragment (TAB-324CQ-F(E)) | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-327CQ | Rabbit Anti-VEGFR2 Recombinant Antibody (TAB-327CQ) | ELISA, WB, Block | Rabbit IgG |
| TAB-327CQ-S(P) | Rabbit Anti-VEGFR2 Recombinant Antibody; scFv Fragment (TAB-327CQ-S(P)) | ELISA, WB, Block | Rabbit scFv |
| TAB-327CQ-F(E) | Rabbit Anti-VEGFR2 Recombinant Antibody; Fab Fragment (TAB-327CQ-F(E)) | ELISA, WB, Block | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-328CQ | Human Anti-VEGFR2 Recombinant Antibody (TAB-328CQ) | ELISA, WB | Human IgG1, κ |
| TAB-328CQ-S(P) | Human Anti-VEGFR2 Recombinant Antibody; scFv Fragment (TAB-328CQ-S(P)) | ELISA, WB, Block | Human scFv |
| TAB-328CQ-F(E) | Human Anti-VEGFR2 Recombinant Antibody; Fab Fragment (TAB-328CQ-F(E)) | ELISA, WB, Block | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2710LY-F(E) | Mouse Anti-VEGFR2 Recombinant Antibody; Fab Fragment (HPAB-2710LY-F(E)) | ELISA, Inhib | Mouse Fab |
| HPAB-2711LY-F(E) | Mouse Anti-VEGFR2 Recombinant Antibody; Fab Fragment (HPAB-2711LY-F(E)) | ELISA, Inhib | Mouse Fab |
| HPAB-2712LY-F(E) | Mouse Anti-VEGFR2 Recombinant Antibody; Fab Fragment (HPAB-2712LY-F(E)) | ELISA, Inhib | Mouse Fab |
| HPAB-2713LY-F(E) | Mouse Anti-VEGFR2 Recombinant Antibody; Fab Fragment (HPAB-2713LY-F(E)) | ELISA, Inhib | Mouse Fab |
| HPAB-2714LY-F(E) | Mouse Anti-VEGFR2 Recombinant Antibody (clone CBL054); Fab Fragment | ELISA, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2710LY | Mouse Anti-VEGFR2 Recombinant Antibody (HPAB-2710LY) | ELISA, Inhib | Mouse IgG |
| HPAB-2711LY | Mouse Anti-VEGFR2 Recombinant Antibody (HPAB-2711LY) | ELISA, Inhib | Mouse IgG |
| HPAB-2712LY | Mouse Anti-VEGFR2 Recombinant Antibody (HPAB-2712LY) | ELISA, Inhib | Mouse IgG |
| HPAB-2713LY | Mouse Anti-VEGFR2 Recombinant Antibody (HPAB-2713LY) | ELISA, Inhib | Mouse IgG |
| HPAB-2714LY | Mouse Anti-VEGFR2 Recombinant Antibody (clone CBL054) | ELISA, Inhib | Mouse IgG |
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