Recombinant Anti-mouse Was Intrabody [+36 GFP]
CAT#: IAB-B076(G)
Recombinant anti-Was intrabody in fusion with +36GFP works within cells and specifically reacts with against the N-terminal domain of Wiskott–Aldrich syndrome protein Was, expressed in CHO cells. The the VH/VL single domain intrabodies are sufficient to knockdown the domain function of target proteins in the cytosol.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
Normal Tissue
Figure 2 High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
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Specifications
- Immunogen
- mouse Wiskott–Aldrich syndrome protein
- Host Species
- mouse
- Type
- scFv-(+36GFP)
- Cell Penetrating Peptide
- +36 GFP
GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.
- Species Reactivity
- mouse
- Applications
- Products can be used for: Western blot; Immunocytochemistry; Functional studies
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- Store at -20°C. Avoid multiple freeze/thaw cycles.
Target
- Alternative Names
- Was; Wiskott–Aldrich syndrome protein
- Gene ID
- 22376
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "WAS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3677z | Mouse Anti-WAS Recombinant Antibody (clone 22F10) | WB, ELISA, FC, IHC | Mouse IgG2a |
| MOB-2231CT | Recombinant Mouse anti-Human WAS Monoclonal antibody (EML2139) | WB | |
| HPAB-0815-CN | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1) | ELISA, IHC | Rabbit IgG |
| VS3-XY1593 | Mouse Anti-WAS Recombinant Antibody (clone 10F1G12) | ELISA, WB, IHC, FC | Mouse IgG2a |
| VS3-XY1594 | Mouse Anti-WAS Recombinant Antibody (clone 4H9E7) | ELISA, WB, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B076(A) | Recombinant Anti-mouse Was Intrabody [(D-Arg)9] | ELISA, WB, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B076(T) | Recombinant Anti-mouse Was Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0627MZ | Chicken Anti-WAS Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3863 | Rabbit Anti-WAS Recombinant Antibody (clone DS3863AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-S(P) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); scFv Fragment | ELISA, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-F(E) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); Fab Fragment | ELISA, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1226 | CytoStream™ Rabbit Anti-WAS Recombinant Antibody (VS13-YC1226) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2484 | Anti-WAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY7892 | Anti-Human WAS Immunohistochemistry Kit | IHC |
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