Recombinant Anti-mouse Was Intrabody [(D-Arg)9] (CAT#: IAB-B076(A))
Recombinant anti-Was intrabody linked to (D-Arg)9 works within cells to bind against the N-terminal domain of Wiskott–Aldrich syndrome protein Was, expressed in CHO cells. The the VH/VL single domain intrabodies are sufficient to knockdown the domain function of target proteins in the cytosol.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
Figure 2 High expression in appendix
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
Specifications
- Immunogen
- mouse Wiskott–Aldrich syndrome protein
- Host Species
- mouse
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- mouse
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western blot; Immunocytochemistry; Functional studies
Product Property
- Purity
- Purity>95% by SDS-PAGE
- Storage
- Store at -20°C. Avoid multiple freeze/thaw cycles.
Target
- Alternative Names
- Was; Wiskott–Aldrich syndrome protein
- Gene ID
- 22376
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3677z | Mouse Anti-WAS Recombinant Antibody (clone 22F10) | WB, ELISA, FC, IHC | Mouse IgG2a |
| MOB-2231CT | Recombinant Mouse anti-Human WAS Monoclonal antibody (EML2139) | WB | |
| HPAB-0815-CN | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1) | ELISA, IHC | Rabbit IgG |
| VS3-XY1593 | Mouse Anti-WAS Recombinant Antibody (clone 10F1G12) | ELISA, WB, IHC, FC | Mouse IgG2a |
| VS3-XY1594 | Mouse Anti-WAS Recombinant Antibody (clone 4H9E7) | ELISA, WB, IHC, FC | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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