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Recombinant Anti-mouse Was Intrabody [(D-Arg)9] (CAT#: IAB-B076(A))

Recombinant anti-Was intrabody linked to (D-Arg)9 works within cells to bind against the N-terminal domain of Wiskott–Aldrich syndrome protein Was, expressed in CHO cells. The the VH/VL single domain intrabodies are sufficient to knockdown the domain function of target proteins in the cytosol.


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Subcellular Location
Figure 1 IF staining of human cell line HEL
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
Normal Tissue
Figure 2 High expression in appendix
Figure 2 High expression in appendix

* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
Figure 4 Testis
Figure 4 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
Figure 5 Lymph node
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS

Specifications

  • Immunogen
  • mouse Wiskott–Aldrich syndrome protein
  • Host Species
  • mouse
  • Type
  • scFv-(D-Arg)9
  • Cell Penetrating Peptide
  • (D-Arg)9
    It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
  • Species Reactivity
  • mouse
  • Applications
  • Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western blot; Immunocytochemistry; Functional studies

Product Property

  • Purity
  • Purity>95% by SDS-PAGE
  • Storage
  • Store at -20°C. Avoid multiple freeze/thaw cycles.

Target

  • Alternative Names
  • Was; Wiskott–Aldrich syndrome protein

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "WAS"

Recombinant Antibody

CAT Product Name Application Type
MOB-3677z Mouse Anti-WAS Recombinant Antibody (clone 22F10) WB, ELISA, FC, IHC Mouse IgG2a
MOB-2231CT Recombinant Mouse anti-Human WAS Monoclonal antibody (EML2139) WB
HPAB-0815-CN Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1) ELISA, IHC Rabbit IgG
VS3-XY1593 Mouse Anti-WAS Recombinant Antibody (clone 10F1G12) ELISA, WB, IHC, FC Mouse IgG2a
VS3-XY1594 Mouse Anti-WAS Recombinant Antibody (clone 4H9E7) ELISA, WB, IHC, FC Mouse IgG2a

Intrabody

CAT Product Name Application Type
IAB-B076(G) Recombinant Anti-mouse Was Intrabody [+36 GFP] WB, ICC, FuncS scFv-(+36GFP)
IAB-B076(T) Recombinant Anti-mouse Was Intrabody [Tat] ICC, Neut, FuncS scFv-Tat

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0627MZ Chicken Anti-WAS Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3863 Rabbit Anti-WAS Recombinant Antibody (clone DS3863AB) ICC, IHC-P, WB Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0815-CN-S(P) Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); scFv Fragment ELISA, IHC Rabbit scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0815-CN-F(E) Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); Fab Fragment ELISA, IHC Rabbit Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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