Recombinant Anti-mouse Was Intrabody [Tat] (CAT#: IAB-B076(T))
Recombinant anti-Was intrabody in fusion with HIV Tat works within cells to bind against the N-terminal domain of Wiskott–Aldrich syndrome protein Was, expressed in CHO cells. The the VH/VL single domain intrabodies are sufficient to knockdown the domain function of target proteins in the cytosol.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
Figure 2 High expression in appendix
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
Specifications
- Immunogen
- mouse Wiskott–Aldrich syndrome protein
- Host Species
- mouse
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- mouse
- Applications
- Can be useful in applications such as: Immunocytochemistry; Neutralization; Functional studies
Product Property
- Purity
- >95% as determined by analysis by RP-HPLC
- Storage
- ≤6 months at 4°C; ≥6 months at -20°C.
Target
- Alternative Names
- Was; Wiskott–Aldrich syndrome protein
- Gene ID
- 22376
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3677z | Mouse Anti-WAS Recombinant Antibody (clone 22F10) | WB, ELISA, FC, IHC | Mouse IgG2a |
| MOB-2231CT | Recombinant Mouse anti-Human WAS Monoclonal antibody (EML2139) | WB | |
| HPAB-0815-CN | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1) | ELISA, IHC | Rabbit IgG |
| VS3-WK20 | Mouse Anti-WAS Recombinant Antibody (clone E10-4B) | WB, IHC, FC | Mouse IgG2a |
| VS3-XY1593 | Mouse Anti-WAS Recombinant Antibody (clone 10F1G12) | ELISA, WB, IHC, FC | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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