Recombinant Anti-mouse Was Intrabody [Tat]
CAT#: IAB-B076(T)
Recombinant anti-Was intrabody in fusion with HIV Tat works within cells to bind against the N-terminal domain of Wiskott–Aldrich syndrome protein Was, expressed in CHO cells. The the VH/VL single domain intrabodies are sufficient to knockdown the domain function of target proteins in the cytosol.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
Normal Tissue
Figure 2 High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
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Specifications
- Immunogen
- mouse Wiskott–Aldrich syndrome protein
- Host Species
- mouse
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- mouse
- Applications
- Can be useful in applications such as: Immunocytochemistry; Neutralization; Functional studies
Product Property
- Purity
- >95% as determined by analysis by RP-HPLC
- Storage
- ≤6 months at 4°C; ≥6 months at -20°C.
Target
- Alternative Names
- Was; Wiskott–Aldrich syndrome protein
- Gene ID
- 22376
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "WAS"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B076(A) | Recombinant Anti-mouse Was Intrabody [(D-Arg)9] | ELISA, WB, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B076(G) | Recombinant Anti-mouse Was Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2231CT | Recombinant Mouse anti-Human WAS Monoclonal antibody (EML2139) | WB | |
| HPAB-0815-CN | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1) | ELISA, IHC | Rabbit IgG |
| VS3-XY1593 | Mouse Anti-WAS Recombinant Antibody (clone 10F1G12) | ELISA, WB, IHC, FC | Mouse IgG2a |
| VS3-XY1594 | Mouse Anti-WAS Recombinant Antibody (clone 4H9E7) | ELISA, WB, IHC, FC | Mouse IgG2a |
| VS7-0425-WR1122 | Mouse Anti-WAS Recombinant Antibody (clone 7B10E4) | IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0627MZ | Chicken Anti-WAS Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3863 | Rabbit Anti-WAS Recombinant Antibody (clone DS3863AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-S(P) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); scFv Fragment | ELISA, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-F(E) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); Fab Fragment | ELISA, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1226 | CytoStream™ Rabbit Anti-WAS Recombinant Antibody (VS13-YC1226) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2484 | Anti-WAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY7892 | Anti-Human WAS Immunohistochemistry Kit | IHC |
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