Mouse Anti-WAS Recombinant Antibody (clone 10F1G12)
CAT#: VS3-XY1593
Clone 10F1G12 is a mouse antibody, which reacts with human WAS protein. This antibody is suitable for WAS detection by ELISA, WB, IHC, FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
Normal Tissue
Figure 2 High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
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Specifications
- Immunogen
- Recombinant fragment of human WAS (a.a. 57-170) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human WAS
- Species Reactivity
- Human
- Clone
- 10F1G12
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000) and Flow Cytometry (1/200-1/400).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B076(A) | Recombinant Anti-mouse Was Intrabody [(D-Arg)9] | ELISA, WB, ICC, FuncS | scFv-(D-Arg)9 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0627MZ | Chicken Anti-WAS Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3863 | Rabbit Anti-WAS Recombinant Antibody (clone DS3863AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-S(P) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); scFv Fragment | ELISA, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-F(E) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); Fab Fragment | ELISA, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1226 | CytoStream™ Rabbit Anti-WAS Recombinant Antibody (VS13-YC1226) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2484 | Anti-WAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY7892 | Anti-Human WAS Immunohistochemistry Kit | IHC |
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