Mouse Anti-WAS Recombinant Antibody (clone 10F1G12) (CAT#: VS3-XY1593)
Clone 10F1G12 is a mouse antibody, which reacts with human WAS protein. This antibody is suitable for WAS detection by ELISA, WB, IHC, FC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/1813_F8_24_cr59f33e96be102_selected.jpg
Figure 2 High expression in appendix
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_1_2_val_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_B_8_5.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4290/12454_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2022/7424_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015285-WAS
Specifications
- Immunogen
- Recombinant fragment of human WAS (a.a. 57-170) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human WAS
- Species Reactivity
- Human
- Clone
- 10F1G12
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000) and Flow Cytometry (1/200-1/400).
Target
- Alternative Names
- Wiskott-Aldrich Syndrome; Thrombocytopenia 1 (X-Linked); Eczema-Thrombocytopenia; WASP; IMD2; Wiskott-Aldrich Syndrome (Eczema-Thrombocytopenia)
- Gene ID
- 7454
- UniProt ID
- P42768
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "WAS"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3677z | Mouse Anti-WAS Recombinant Antibody (clone 22F10) | WB, ELISA, FC, IHC | Mouse IgG2a |
| MOB-2231CT | Recombinant Mouse anti-Human WAS Monoclonal antibody (EML2139) | WB | |
| HPAB-0815-CN | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1) | ELISA, IHC | Rabbit IgG |
| VS3-WK20 | Mouse Anti-WAS Recombinant Antibody (clone E10-4B) | WB, IHC, FC | Mouse IgG2a |
| VS3-XY1594 | Mouse Anti-WAS Recombinant Antibody (clone 4H9E7) | ELISA, WB, IHC, FC | Mouse IgG2a |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B076(A) | Recombinant Anti-mouse Was Intrabody [(D-Arg)9] | ELISA, WB, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B076(G) | Recombinant Anti-mouse Was Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B076(T) | Recombinant Anti-mouse Was Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0627MZ | Chicken Anti-WAS Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3863 | Rabbit Anti-WAS Recombinant Antibody (clone DS3863AB) | ICC, IHC-P, WB | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-S(P) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); scFv Fragment | ELISA, IHC | Rabbit scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0815-CN-F(E) | Rabbit Anti-WAS Recombinant Antibody (clone CBCNT1); Fab Fragment | ELISA, IHC | Rabbit Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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