Recombinant Anti-human CD8A Antibody Fab Fragment
CAT#: MOB-184-F(E)
Recombinant Mouse Antibody Fab Fragment binds selectively to Human CD8A, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1373_A3_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1492_D3_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1377_A3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12/97_A_7_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153563-CD8A
❮
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Specifications
- Immunogen
- Human CD8a molecule
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Fab
- Specificity
- Tested positive against native Human CD8a molecule
- Species Reactivity
- Human
- Applications
- Formats of immunological tests: Western blot; Enzyme-linked Immunosorbent Assay; Functional Study
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- ≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C
Target
- Alternative Names
- CD8A; CD8a molecule; CD8; MAL; p32; Leu2; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte different
- Gene ID
- 925
- UniProt ID
- P01732
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NEUT-429CQ | Mouse Anti-CD8A Recombinant Antibody (clone CT-8) | Neut, FC, IHC, IHC-Fr, IHC-P, IP, ICC | Mouse IgG1, κ |
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