Recombinant Human Anti-IgG Fc Antibody scFv Fragment

CAT#: MHH-548-S(P)

Recombinant Human Antibody scFv Fragment specifically reacts with IgG Fc, expressed in E. coli.

Specifications

  • Immunogen
  • Immunoglobulin G Fc
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • scFv
  • Specificity
  • Tested positive against native Immunoglobulin G Fc
  • Applications
  • Products can be used for: Western blot; Enzyme-linked Immunosorbent Assay; Immunohistochemistry; Functional Study

Product Property

  • Purity
  • >97%, by SDS-PAGE under reducing conditions
  • Storage
  • Store at -20°C. Avoid multiple freeze/thaw cycles.

Target

  • Alternative Names
  • IgG Fc; Immunoglobulin G Fc
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Breast cancer biomarkers at key points during disease progression
High purity and affinity
The high purity and affinity of this scFv fragment streamlined our protein interaction studies, providing clear and interpretable data.
Breast cancer biomarkers at key points during disease progression
Low cross-reactivity
This reagent's low cross-reactivity with IgG from other species was crucial for our cross-species comparison studies. It has outperformed other reagents we've used.
Breast cancer biomarkers at key points during disease progression
Great for WB
We achieved excellent Western blot results using this scFv fragment. However, we had to optimize the blocking conditions a bit more than usual to get the best results.

Q&As

  1. Can this single-chain variable fragment (scFv) be utilized in enzyme-linked immunosorbent assay (ELISA) procedures?

    A: Yes, the Recombinant Human Anti-IgG Fc Antibody scFv Fragment is suitable for use in ELISA to detect human IgG.

  2. Is this scFv fragment compatible with Western blotting?

    A: Yes, it can be utilized in Western blotting applications to detect the Fc region of human IgG proteins.

  3. Can the scFv fragment be conjugated to labels such as enzymes or fluorophores?

    A: Yes, this scFv fragment can be conjugated to various labels including enzymes, fluorophores, and biotin for diverse applications.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MHH-548-S(P), RRID: AB_3111460)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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