Anti-Human TNFRSF4 Recombinant Antibody (TAB-452CQ) (CAT#: TAB-452CQ)
Recombinant monoclonal antibody to TNFRSF4. This antibody is a humanized monoclonal antibody that binds specifically to TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily Member 4), also known as OX40.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
SDS-PAGE
Figure 1 TNFRSF4 Recombinant Antibody (TAB-452CQ) in SDS-PAGE
SDS-PAGE analysis of TAB-452CQ in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
SEC-HPLC
Figure 2 TNFRSF4 Recombinant Antibody (TAB-452CQ) in HPLC
The purity of TAB-452CQ was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8x300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV280 nm
Injection: 25 µl
WB
Figure 3 TNFRSF4 Recombinant Antibody (TAB-452CQ) in WB
Western blot analysis was performed by loading 5 µg (lane 1) and 2 µg (lane 2) recombinant human OX40/TNFRSF4 Fc Chimera (3388-OX-050) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with TNFRSF4 Therapeutic Antibody (TAB-452CQ) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized and chimeric
- Type
- IgG1, κ
- Specificity
- Human TNFRSF4
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Functional Studies
- Conjugate
- Unconjugated
- Related Disease
- Advanced Solid Tumors
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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