What is the biological function of the target VEGF?

VEGF is a heparin-binding protein that induces proliferation and migration of vascular endothelial cells. It is essential for physiological and pathological angiogenesis, and its disruption results in abnormal embryonic blood vessel formation.

What affinity was achieved for this ubiquitin scaffold?

After affinity maturation, ubiquitin variants were identified having affinities (KD=50 nM) for VEGF. This affinity was determined by biosensor technology, demonstrating the potential of ubiquitin as a scaffold for generating specific binders.

Ubiquitin scaffold protein anti-Human VEGF

CAT#: SUB-L210

The ubiquitin that bound VEGF was isolated from the library using Phage display techonology. After affinity maturation, ubiquitin variants were identified having affinities (KD=50 nM) for VEGF as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

Gene Expression

Normal Tissue Figure 1 Cerebral cortex

Neuronal cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA

❮

❯

Specifications

Scaffold Name
Ubiquitin

Origin
Ubiquitin-like proteins

Core Structure
α/β (76)

Variable Regions
8 aa

Target
VEGF

Species Reactivity
Human

Expression Host
E. coli

Affinity Constant
50 nM

Applications
ELISA; IHC; Microscopy; FC; WB; FuncS

Target

Alternative Names
ubiquitin-like proteins; ubiquitin; VPF; VEGF; MVCD1

Gene ID
7422

UniProt ID
P15692

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Breast cancer biomarkers at key points during disease progression

Strong VEGF Binding

The affinity of this ubiquitin variant for VEGF is excellent, measured at approximately 50 nM. It effectively targets the growth factor in our angiogenesis assays, allowing us to study proliferation and migration of vascular endothelial cells.

Small Stable Scaffold

We chose this ubiquitin scaffold for its small size and stability. It works well in ELISA and functional studies, acting as a robust probe for detecting VEGF presence in various tissue samples related to tumor progression.

Q&As

What is the biological function of the target VEGF?

A: VEGF is a heparin-binding protein that induces proliferation and migration of vascular endothelial cells. It is essential for physiological and pathological angiogenesis, and its disruption results in abnormal embryonic blood vessel formation.
What affinity was achieved for this ubiquitin scaffold?

A: After affinity maturation, ubiquitin variants were identified having affinities (KD=50 nM) for VEGF. This affinity was determined by biosensor technology, demonstrating the potential of ubiquitin as a scaffold for generating specific binders.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Datasheet

Datasheet - SUB-L210.pdf

MSDS

MSDS - SUB-L210.pdf

COA

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