Anti-ABCB1 Antibody Prodrug, Protease Activated (VS-1025-YC138)
CAT#: VS-1025-YC138
The Anti-ABCB1 Antibody Prodrug, Protease Activated (VS-1025-YC138) is an antibody prodrug comprised of a recombinant human antibody targeting human ABCB1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to ABCB1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to ABCB1 effectively. ABCB1 (P-glycoprotein/MDR1) is an efflux pump that acts as the primary mechanism for multidrug resistance (MDR) in many cancers. By actively transporting various chemotherapeutic agents out of the cell, its overexpression reduces drug efficacy, leading to treatment failure.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Hep G2, RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human ABCB1
- Target
- ABCB1
- Species Reactivity
- Human
- Isotype
- IgG1, kappa
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
- Alternative Names
- ABC20; CD243; CLCS; GP170; MDR1; P-GP; PGY1
- Gene ID
- 5243
- UniProt ID
- P08183
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Datasheet
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| PABC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219) | WB, IF, IHC, FC, FuncS | Mouse IgG |
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| PFBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); Fab Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); scFv Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
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| MOB-1496CT | Mouse Anti-ABCB1 Recombinant Antibody (clone 5F4) | FC, IF | Mouse IgG2a |
| CAT | Product Name | Application | Type |
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| NEUT-001CQ | Mouse Anti-ABCB1 Recombinant Antibody (clone UIC2) | FC, Block, IP, IHC | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
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| MOR-0009 | Hi-Affi™ Rabbit Anti-ABCB1 Recombinant Antibody (clone DS9AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| PABC-444 | Human Anti-ABCB1 Recombinant Antibody (PABC-444) | FuncS, FC, Block, IP, IHC | Human IgG1, κ |
| CAT | Product Name | Application | Type |
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| PFBC-444 | Human Anti-ABCB1 Recombinant Antibody Fab Fragment (PFBC-444) | FC, Block, IP, IHC | Human Fab |
| CAT | Product Name | Application | Type |
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| PSBC-444 | Human Anti-ABCB1 Recombinant Antibody scFv Fragment (PSBC-444) | FC, Block, IP, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
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| HPAB-M0001-YC-S(P) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); scFv Fragment | IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0001-YC-F(E) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); Fab Fragment | IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
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| VS-0325-XY6 | Anti-ABCB1 Immunohistochemistry Kit | IHC |
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| VS-0525-XY16 | Anti-Mouse ABCB1 Immunohistochemistry Kit | IHC |
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| VS-0525-XY15 | Anti-Human ABCB1 Immunohistochemistry Kit | IHC |
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| VS-0525-XY17 | Anti-Goat ABCB1 Immunohistochemistry Kit | IHC |
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