Human Anti-ABCB1 Recombinant Antibody (PABC-444)
CAT#: PABC-444
This product is a recombinant human antibody that can recognize ABCB1. This antibody is a human ABCB1-specific inhibitory antibody. This antibody recognizes a conformational epitope of ABCB1 that involves residues from TM1 and TM2 and the extracellular loops EL1, EL3, and EL4.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Hep G2, RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
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Specifications
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Human IgG1, κ
- Specificity
- Human ABCB1
- Species Reactivity
- Human
- Applications
- FuncS, FC, Block, IP, IHC
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Functional Assay, Flow Cytometry, Blocking, Immunoprecipitation and Immunohistochemistry. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ABCB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22 | Recombinant Anti-human ABCB1 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-22 | Recombinant Human Anti-human ABCB1 Antibody | FC, Neut, Funcs | IgG |
| MRO-1138-CN | Rabbit Anti-ABCB1 Recombinant Antibody (clone CBACN-413) | WB, IHC | Rabbit IgG |
| HPAB-M0001-YC | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6) | IHC | Mouse IgG |
| VS3-CJ488 | Rabbit Anti-ABCB1 Recombinant Antibody (VS3-CJ488) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-F(E) | Recombinant Anti-human ABCB1 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| MHH-22-F(E) | Recombinant Human Anti-human ABCB1 Antibody Fab Fragment | WB, FC, FuncS | Fab |
| PFBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); Fab Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse IgG |
| PFBC-444 | Human Anti-ABCB1 Recombinant Antibody Fab Fragment (PFBC-444) | FC, Block, IP, IHC | Human Fab |
| HPAB-M0001-YC-F(E) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); Fab Fragment | IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-S(P) | Recombinant Anti-human ABCB1 Antibody scFv Fragment | Neut, IF, FuncS | scFv |
| MHH-22-S(P) | Recombinant Human Anti-human ABCB1 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| PSBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); scFv Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse scFv |
| PSBC-444 | Human Anti-ABCB1 Recombinant Antibody scFv Fragment (PSBC-444) | FC, Block, IP, IHC | Mouse scFv |
| HPAB-M0001-YC-S(P) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); scFv Fragment | IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-001CQ | Mouse Anti-ABCB1 Recombinant Antibody (clone UIC2) | FC, Block, IP, IHC | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0009 | Hi-Affi™ Rabbit Anti-ABCB1 Recombinant Antibody (clone DS9AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY6 | Anti-ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY16 | Anti-Mouse ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY15 | Anti-Human ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY17 | Anti-Goat ABCB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC423 | Recombinant Anti-ABCB1 Vesicular Antibody, EV Displayed (VS-0425-YC423) | Block, ELISA, FC, Cell-uptake |
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