Mouse Anti-ABCB1 Recombinant Antibody (clone 5F4) (CAT#: MOB-1496CT)
Recombinant Mouse monoclonal antibody to Human ABCB1, expressed in mammalian cells.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
Figure 6 RNA cell line category: Cell line enhanced (Hep G2, RH-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
Specifications
- Immunogen
- Tissue / cell preparation (Human squamous lung cancer cell line SW-1573/500).
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- P-glycoprotein - 170 Kda (External Epitope) The 5F4 monoclonal antibody recognizes an epitope of the mdr1 encoded P-glycoprotein located on the exterior surface of the plasma membrane. 5F4 does not cross-react with the mdr3 gene product.
- Species Reactivity
- Human
- Clone
- 5F4
- Applications
- FC, IF
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Concentration
- 0.1 mg/ml
- Buffer
- Preservative: 0.1% Sodium Azide Constituents: 1% BSA, PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ABCB1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; multidrug resistance protein 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance;
- Gene ID
- 5243
- UniProt ID
- P08183
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-F(E) | Recombinant Anti-human ABCB1 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| MHH-22-F(E) | Recombinant Human Anti-human ABCB1 Antibody Fab Fragment | WB, FC, FuncS | Fab |
| PFBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); Fab Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse IgG |
| PFBC-444 | Human Anti-ABCB1 Recombinant Antibody Fab Fragment (PFBC-444) | FC, Block, IP, IHC | Human Fab |
| HPAB-M0001-YC-F(E) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); Fab Fragment | IHC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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