Mouse Anti-ABCG2 Recombinant Antibody (ZG-0258F)
CAT#: ZG-0258F
This product is a mouse antibody that recognizes ABCG2.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-ABCG2 Recombinant Antibody (ZG-0258F) in WB.
Western blot analysis was performed using ABCG2 monoclonal antibodies against HepG2 (1), Cos7 (2), Jurkat (3) and NIH/3T3 (4) cell lysates.
IF
Figure 2 Mouse Anti-ABCG2 Recombinant Antibody (ZG-0258F) in IF.
The ABCG2 monoclonal antibody (green) was used for immunofluorescence analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA
Figure 3 Mouse Anti-ABCG2 Recombinant Antibody (ZG-0258F) in ELISA.
ELISA analysis of ZG-0258F was performed by coating with human ABCG2 Protein.
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Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37299/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_B_7_5.jpg
Normal Tissue
Figure 4 Small intestine
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_4_2.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, MCF7, OE19, RPMI-8226, SuSa)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118777-ABCG2
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Specifications
- Immunogen
- Recombinant fragment of human ABCG2 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ABCG2
- Species Reactivity
- Human, Mouse, Monkey
- Applications
- Western Blot, Immunofluorescence, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunofluorescence: 1/200-1/1000.
ELISA: 1/10000
Target
- Alternative Names
- ATP Binding Cassette Subfamily G Member 2 (Junior Blood Group); ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (Junior Blood Group); Placenta-Specific ATP-Binding Cassette Transporter; Mitoxantrone Resistance-Associated Protein; Breast Cancer Resistance Protein; Urate Exporter; CDw338; BCRP1; ABCP; BCRP; MXR; Multi Drug Resistance Efflux Transport ATP-Binding Cassette Sub-Family G (WHITE) Member 2; ATP-Binding Cassette, Sub-Family G (WHITE), Member 2; ATP-Binding Cassette Sub-Family G Member 2; ABCG2; ABCP; BCRP; BCRP1; MXR; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; CD antigen CD338
- Gene ID
- 9429
- UniProt ID
- Q9UNQ0
REVIEWS AND Q&AS
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Strong Western Blot Signal
We used this mouse antibody to detect ABCG2 in human cell lysates. Following the recommended dilution of 1:1000, we obtained a very strong and specific signal. It is a great tool for studying ATP-binding cassette transporters in cancer resistance.
Versatile For IF And ELISA
This antibody is quite versatile. We have used it for both Immunofluorescence at 1:500 and ELISA at 1:10000. In both applications, it performed reliably, allowing us to visualize and quantify the Breast Cancer Resistance Protein in our samples.
Q&As
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What is the recommended dilution for Immunofluorescence?
A: For Immunofluorescence (IF) applications, we recommend a dilution range of 1/200 to 1/1000. This mouse antibody recognizes ABCG2 (Breast Cancer Resistance Protein) in Human samples. It is also validated for Western Blot and ELISA.
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What is the function of the ABCG2 protein?
A: ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. It functions as a xenobiotic transporter and may play a major role in multi-drug resistance. It is also known as the breast cancer resistance protein (BCRP).
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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