Mouse Anti-ABCG2 Recombinant Antibody (ZG-0258F)
CAT#: ZG-0258F
This product is a mouse antibody that recognizes ABCG2.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-ABCG2 Recombinant Antibody (ZG-0258F) in WB.
Western blot analysis was performed using ABCG2 monoclonal antibodies against HepG2 (1), Cos7 (2), Jurkat (3) and NIH/3T3 (4) cell lysates.
IF
Figure 2 Mouse Anti-ABCG2 Recombinant Antibody (ZG-0258F) in IF.
The ABCG2 monoclonal antibody (green) was used for immunofluorescence analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA
Figure 3 Mouse Anti-ABCG2 Recombinant Antibody (ZG-0258F) in ELISA.
ELISA analysis of ZG-0258F was performed by coating with human ABCG2 Protein.
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Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37299/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_B_7_5.jpg
Normal Tissue
Figure 4 Small intestine
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_4_2.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54719/135304_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, BEWO, MCF7, OE19, RPMI-8226, SuSa)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118777-ABCG2
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Specifications
- Immunogen
- Recombinant fragment of human ABCG2 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ABCG2
- Species Reactivity
- Human, Mouse, Monkey
- Applications
- Western Blot, Immunofluorescence, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunofluorescence: 1/200-1/1000.
ELISA: 1/10000
Target
- Alternative Names
- ATP Binding Cassette Subfamily G Member 2 (Junior Blood Group); ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (Junior Blood Group); Placenta-Specific ATP-Binding Cassette Transporter; Mitoxantrone Resistance-Associated Protein; Breast Cancer Resistance Protein; Urate Exporter; CDw338; BCRP1; ABCP; BCRP; MXR; Multi Drug Resistance Efflux Transport ATP-Binding Cassette Sub-Family G (WHITE) Member 2; ATP-Binding Cassette, Sub-Family G (WHITE), Member 2; ATP-Binding Cassette Sub-Family G Member 2; ABCG2; ABCP; BCRP; BCRP1; MXR; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; CD antigen CD338
- Gene ID
- 9429
- UniProt ID
- Q9UNQ0
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOR-0016 | Hi-Affi™ Rabbit Anti-ABCG2 Recombinant Antibody (clone DS16AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| HPAB-M0002-YC-S(P) | Mouse Anti-ABCG2 Recombinant Antibody; scFv Fragment (HPAB-M0002-YC-S(P)) | ELISA, WB | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0002-YC-F(E) | Mouse Anti-ABCG2 Recombinant Antibody; Fab Fragment (HPAB-M0002-YC-F(E)) | ELISA, WB | Mouse Fab |
| PFBS-0024 | Mouse Anti-ABCG2 Recombinant Antibody (Clone 5D3); Fab Fragment | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC6 | CytoStream™ Rabbit Anti-ABCG2 Recombinant Antibody (VS13-YC6) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC483 | Recombinant Anti-ABCG2 Vesicular Antibody, EV Displayed (VS-0425-YC483) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY41 | Anti-ABCG2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY42 | Anti-Mouse ABCG2 Immunohistochemistry Kit | IHC |
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