SmartAb™ Recombinant Anti-ACVR2B pH-dependent Antibody (VS-0825-YC208)
CAT#: VS-0825-YC208
The anti-ACVR2B pH-dependent antibody (VS-0825-YC208) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human ACVR2B. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for ACVR2B under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from ACVR2B at pH 6.0. This pH-sensitive antibody retains its binding affinity on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. ACVR2B (Activin Receptor Type-2B) is a receptor for activin and myostatin, which are members of the TGF-β superfamily and are key regulators of muscle growth. Antagonizing ACVR2B is a therapeutic strategy being explored for muscle wasting diseases.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of cell line A-431
Immunofluorescent staining of cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/115/672_E2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of cell line U-2 OS
Immunofluorescent staining of cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/115/663_E2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of cell line U-251 MG
Immunofluorescent staining of cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/115/646_E2_1_blue_red_green.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3804/10580_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3804/10580_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25% Spermatogonia cells
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3804/10580_A_4_6.jpg
Normal Tissue
Figure 7 Placenta
Decidual cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3804/10580_A_2_7.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3804/10580_A_8_8.jpg
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human ACVR2B
- Species Reactivity
- Human
- Applications
- FC
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
- Alternative Names
- Activin A Receptor Type 2B; Activin A Receptor, Type IIB; EC 2.7.11.30; ActR-IIB; Activin Receptor Type IIB; Activin Receptor Type-2B; EC 2.7.11; ACTRIIB; HTX4
- Gene ID
- 93
- UniProt ID
- Q13705
- Long Name
- Activin A Receptor Type 2B
- Protein Refseq
- NP_000591.1; NP_000591.1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-50-sdAb | Recombinant Anti-Human ACVR2B VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
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| TAB-012CL | Human Anti-ACVR2B Recombinant Antibody (TAB-012CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0064 | Hi-Affi™ Rabbit Anti-ACVR2B Recombinant Antibody (clone DS64AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-FY-F(E) | Human Anti-ACVR2B Recombinant Antibody; Fab Fragment (HPAB-0038-FY-F(E)) | ELISA | Human Fab |
| HPAB-0575-WJ-F(E) | Human Anti-ACVR2B Recombinant Antibody; Fab Fragment (HPAB-0575-WJ-F(E)) | ELISA | Human Fab |
| HPAB-0576-WJ-F(E) | Human Anti-ACVR2B Recombinant Antibody; Fab Fragment (HPAB-0576-WJ-F(E)) | ELISA | Human Fab |
| HPAB-0577-WJ-F(E) | Human Anti-ACVR2B Recombinant Antibody; Fab Fragment (HPAB-0577-WJ-F(E)) | ELISA | Human Fab |
| HPAB-0578-WJ-F(E) | Human Anti-ACVR2B Recombinant Antibody; Fab Fragment (HPAB-0578-WJ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0010-YC | Human Anti-ACVR2B Recombinant Antibody (HPAB-N0010-YC) | FC | Human IgG |
| HPAB-0575-WJ | Human Anti-ACVR2B Recombinant Antibody (HPAB-0575-WJ) | ELISA | Human IgG |
| ZG-0132U | Rabbit Anti-ACVR2B Recombinant Antibody (clone 7B10) | ELISA, IHC | Rabbit IgG |
| VS3-FY1964 | Rabbit Anti-ACVR2B Recombinant Antibody (clone R01-2I1) | WB | Rabbit IgG |
| VS-0824-YJ24 | Human Anti-ACVR2B Recombinant Antibody (clone CBA103) | IA | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0010-YC-S(P) | Human Anti-ACVR2B Recombinant Antibody scFv Fragment (HPAB-N0010-YC-S(P)) | FC | Human scFv |
| HPAB-0575-WJ-S(P) | Human Anti-ACVR2B Recombinant Antibody; scFv Fragment (HPAB-0575-WJ-S(P)) | ELISA | Human scFv |
| HPAB-0576-WJ-S(P) | Human Anti-ACVR2B Recombinant Antibody; scFv Fragment (HPAB-0576-WJ-S(P)) | ELISA | Human scFv |
| HPAB-0577-WJ-S(P) | Human Anti-ACVR2B Recombinant Antibody; scFv Fragment (HPAB-0577-WJ-S(P)) | ELISA | Human scFv |
| HPAB-0578-WJ-S(P) | Human Anti-ACVR2B Recombinant Antibody; scFv Fragment (HPAB-0578-WJ-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY31 | Human Anti-ACVR2B scFv-Fc Chimera (VS-0425-FY31) | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC316 | Recombinant Anti-ACVR2B Vesicular Antibody, EV Displayed (VS-0425-YC316) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY140 | Anti-ACVR2B Immunohistochemistry Kit | IHC |
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