Rabbit Anti-ADA Recombinant Antibody (VS13-YC22)
CAT#: VS13-YC22
The Rabbit Anti-ADA Recombinant Antibody is specific for human and rat ADA. ADA encodes an enzyme responsible for the hydrolysis of adenosine to inosine, with mutations linked to diseases like SCID, causing B and T lymphocyte dysfunction and impaired immunity, as well as congenital hemolytic anemia when enzyme levels are elevated. The anti-ADA antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/26_D7_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/27_D7_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/28_D7_1_blue_red_green.jpg
Normal Tissue
Figure 4 Duodenum
Enterocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes - Microvilli
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/162544_A_7_2.jpg
Normal Tissue
Figure 5 Testis
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/162544_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196839-ADA
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Specifications
- Immunogen
- Recombinant human ADA.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ADA
- Species Reactivity
- Human, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- NONE
- Related Disease
- Immunodeficiency disease (SCID)
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Target
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Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ADA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1230z | Mouse Anti-ADA Recombinant Antibody (clone 20C10) | FC, IHC, WB | Mouse IgG2b |
| MRO-215-MZ | Mouse Anti-ADA Recombinant Antibody (clone ADA-1) | RIA, WB, IP | Mouse IgG |
| MOB-0430MZ | Recombinant Mouse Anti-Escherichia coli ada Antibody (clone BEB-2) | IP, RIA, WB | Mouse antibody |
| VS3-QX22 | Mouse Anti-ADA Recombinant Antibody (clone 608CT2.1.3) | WB | Mouse IgG1 |
| VS3-CJ221 | Mouse Anti-ADA Recombinant Antibody (VS3-CJ221) | WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0067 | Hi-Affi™ Rabbit Anti-ADA Recombinant Antibody (clone DS67AB) | ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC242 | AbPlus™ Anti-ADA Magnetic Beads (VS-0724-YC242) | IP, Protein Purification | |
| VS-0724-YC262 | AbPlus™ Anti-ADA Magnetic Beads (VS-0724-YC262) | IP, Protein Purification |
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