Human Anti-ADAMTS13 Recombinant Antibody (clone I-9) (CAT#: FAMAB-0029JF)
Recombinant monoclonal antibody can sepecially bind to human progesterone. This anti-ADAMTS13 scFv antibody I-9 is useful in the treatment of patients with acquired thrombotic thrombocytopenic purpura (TTP). The antibody has been tested in some applications including Western blot (WB), ELISA, Immunohistochemistry (IHC), Inhibition and Functional assay.
Specific Inquiry
WB
Figure 1 Domain organization of ADAMTS13 and a map of the ADAMTS13 fragments (I, II, III, IV, V and VI) expressed in E. coli.
Indicated are the signal peptide (S), the propeptide (P), the catalytic, the disintegrin-like, the cysteine-rich (Cys), the spacer domain, the two CUB domains as well as the 8 TSP type 1 repeats.
Klaus, C., Plaimauer, B., Studt, J. D., Dorner, F., Lämmle, B., Mannucci, P. M., & Scheiflinger, F. (2004). Epitope mapping of ADAMTS13 autoantibodies in acquired thrombotic thrombocytopenic purpura. Blood, 103(12), 4514-4519.
Inhib
Figure 2 ADAMTS13 inhibitor titer.
Titer is shown in the acute phase in 32 surviving TMA patients.
Ferrari, S., Scheiflinger, F., Rieger, M., Mudde, G., Wolf, M., Coppo, P., ... & Mira, J. P. (2007). Prognostic value of anti-ADAMTS13 antibody features (Ig isotype, titer, and inhibitory effect) in a cohort of 35 adult French patients undergoing a first episode of thrombotic microangiopathy with undetectable ADAMTS13 activity. Blood, 109(7), 2815-2822.
ELISA
Figure 3 ADAMTS13 inhibitor and anti-ADAMTS13 IgG/IgM/IgA in a cohort of 35 patients with acute TMA.
Each TMA patient is represented by a symbol. Black circles are for patients with IgG, gray circles for patients with IgA, white circles for patients with IgM, and black triangles for patients with no detectable IgG/IgM/IgA.
Ferrari, S., Scheiflinger, F., Rieger, M., Mudde, G., Wolf, M., Coppo, P., ... & Mira, J. P. (2007). Prognostic value of anti-ADAMTS13 antibody features (Ig isotype, titer, and inhibitory effect) in a cohort of 35 adult French patients undergoing a first episode of thrombotic microangiopathy with undetectable ADAMTS13 activity. Blood, 109(7), 2815-2822.
IHC
Figure 4 ADAMTS13 expression in normal renal cortex and in patients with tubulopathy.
ADAMTS13 antigen was detected in all tubular cells (both proximal and distal) in normal renal cortex.
Manea, M., Tati, R., Karlsson, J., Békássy, Z. D., & Karpman, D. (2010). Biologically active ADAMTS13 is expressed in renal tubular epithelial cells. Pediatric Nephrology, 25(1), 87.
FuncS
Figure 5 ADAMTS13 detected in cultured renal tubular epithelial cells.
Results were run three times using cells from two experiments and are presented as mean and range.
Manea, M., Tati, R., Karlsson, J., Békássy, Z. D., & Karpman, D. (2010). Biologically active ADAMTS13 is expressed in renal tubular epithelial cells. Pediatric Nephrology, 25(1), 87.
Specifications
- Host Species
- Human
- Derivation
- Human phage display scFv library
- Type
- Human IgG
- Specificity
- Human ADAMTS13
- Species Reactivity
- Human
- Clone
- I-9
- Applications
- WB, ELISA, IHC, Inhib, FuncS
- Related Disease
- Thrombotic thrombocytopenic purpura
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The ADAMTS13 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Functional Assay, Inhibition.
Target
- Alternative Names
- ADAM Metallopeptidase With Thrombospondin Type 1 Motif, 13; A Disintegrin-Like And Metalloprotease (Reprolysin Type) With Thrombospondin Type 1 Motif, 13; Von Willebrand Factor-Cleaving Protease; VWF-Cleaving Protease; ADAM-TS13; ADAMTS-13; C9orf8; VWF-CP; EC 3.4.24.14; EC 3.4.24.82; EC 3.4.24.87; ADAM-TS 13; VWFCP
- Gene ID
- 11093
- UniProt ID
- Q76LX8
- Function
- Inhibitor
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1290z-F(E) | Recombinant Anti-Human ADAMTS13 Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| HPAB-1567LY-F(E) | Human Anti-ADAMTS13 Recombinant Antibody; Fab Fragment (HPAB-1567LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-1568LY-F(E) | Human Anti-ADAMTS13 Recombinant Antibody; Fab Fragment (HPAB-1568LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-1569LY-F(E) | Human Anti-ADAMTS13 Recombinant Antibody; Fab Fragment (HPAB-1569LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-1570LY-F(E) | Human Anti-ADAMTS13 Recombinant Antibody; Fab Fragment (HPAB-1570LY-F(E)) | ELISA, Inhib | Human Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0072 | Hi-Affi™ Rabbit Anti-ADAMTS13 Recombinant Antibody (clone DS72AB) | WB, FC | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0202-YC-S(P) | Human Anti-ADAMTS13 Recombinant Antibody; scFv Fragment (HPAB-0202-YC-S(P)) | ELISA | Human scFv |
| HPAB-1594LY-S(P) | Human Anti-ADAMTS13 Recombinant Antibody; scFv Fragment (HPAB-1594LY-S(P)) | ELISA, Inhib | Human scfv |
| HPAB-0201-WJ-S(P) | Mouse Anti-ADAMTS13 Recombinant Antibody; scFv Fragment (HPAB-0201-WJ-S(P)) | ELISA, IP | Mouse scFv |
| HPAB-0202-WJ-S(P) | Mouse Anti-ADAMTS13 Recombinant Antibody; scFv Fragment (HPAB-0202-WJ-S(P)) | ELISA, IP | Mouse scFv |
| FAMAB-0029JF-S(P) | Human Anti-ADAMTS13 Recombinant Antibody (clone I-9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1560LY | Human Anti-ADAMTS13 Recombinant Antibody (HPAB-1560LY) | Inhib, ELISA | Human IgG |
| HPAB-1561LY | Human Anti-ADAMTS13 Recombinant Antibody (HPAB-1561LY) | Inhib, ELISA | Human IgG |
| HPAB-1562LY | Human Anti-ADAMTS13 Recombinant Antibody (HPAB-1562LY) | Inhib, ELISA | Human IgG |
| HPAB-1563LY | Human Anti-ADAMTS13 Recombinant Antibody (HPAB-1563LY) | Inhib, ELISA | Human IgG |
| HPAB-1564LY | Human Anti-ADAMTS13 Recombinant Antibody (HPAB-1564LY) | Inhib, ELISA | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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