Rabbit Anti-AGT Recombinant Antibody (VS3-CJ223)
CAT#: VS3-CJ223
This product is a rabbit antibody that recognizes AGT.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct positivity in plasma, glandular cells were negative.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25798/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25798/151642_B_8_5.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25798/151642_A_8_4.jpg
Normal Tissue
Figure 4 Kidney
Proximal tubules (cell body) Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25798/151642_A_8_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (Hep G2, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135744-AGT
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AGT
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- ANHU; SERPINA8; Hflt1
- Gene ID
- 183
- UniProt ID
- P01019
- Sequence Similarities
- Belongs to the serpin family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine (PubMed:17138938).
The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor (PubMed:17138938).
In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1 (PubMed:12045255).
Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (PubMed:4322742, PubMed:10969042).
Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4 (PubMed:10969042, PubMed:11815627).
Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4 (PubMed:10969042, PubMed:11815627).
Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin) (PubMed:15283675).
The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.
- Protein Refseq
- NP_000020.1
- Function
- Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
Angiotensin-2: Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed:10619573, PubMed:17138938).
Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed:1567413).
Also binds the DEAR/FBXW7-AS1 receptor (By similarity).
Angiotensin-3: Stimulates aldosterone release.
Angiotensin 1-7: Is a ligand for the G-protein coupled receptor MAS1 (By similarity).
Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AGT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-008 | Mouse Anti-AGT Recombinant Antibody (clone mAb131); Fab Fragment | WB, IF, FuncS | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-008 | Mouse Anti-AGT Recombinant Antibody (clone mAb131); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0620MZ | Mouse Anti-AGT Recombinant Antibody (clone CHO/LB/5I) | ELISA | Mouse IgG2a |
| MRO-0097-CN | Rabbit Anti-AGT Recombinant Antibody (clone CBACN-031) | WB, IF, IP | Rabbit IgG |
| ZG-0266F | Mouse Anti-AGT Recombinant Antibody (ZG-0266F) | WB, ELISA | Mouse IgG |
| VS3-XY31 | Mouse Anti-AGT Recombinant Antibody (clone 1B1) | ELISA, WB | Mouse IgG2b |
| VS3-FY47 | Recombinant Rabbit Anti-Agt Antibody (clone R04-8F8) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0103 | Hi-Affi™ Rabbit Anti-AGT Recombinant Antibody (clone DS103AB) | FC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC582 | AbPlus™ Anti-AGT Magnetic Beads (VS-0724-YC582) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY60 | Anti-AGT Immunohistochemistry Kit | IHC | |
| VS-0525-XY226 | Anti-Mouse AGT Immunohistochemistry Kit | IHC | |
| VS-0525-XY225 | Anti-Human AGT Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC435 | Recombinant Anti-AGT Vesicular Antibody, EV Displayed (VS-0425-YC435) | ELISA, FC, Cell-uptake |
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