Rabbit Anti-ANPEP Recombinant Antibody (clone R07-2G8) (CAT#: VS3-FY1614)
The R07-2G8 recombinant monoclonal antibody has been developed to detect human ANPEP. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. It is advised to dilute the sample for improved detectability by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The specificity of the CD13 antibody was confirmed by western blot analysis of CD13 in rat brain lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
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WB
Figure 1 Anti-ANPEP Antibody (VS3-FY1614) in WB.
The CD13 expression in rat Brain lysates were detected using the anti-ANPEP/CD13 by western blot assay.
IHC
Figure 2 Anti-ANPEP Antibody (VS3-FY1614) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-CD13 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human CD13
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ANPEP
- Species Reactivity
- Human, Rat
- Clone
- R07-2G8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 110 kDa, while the observed molecular weight stands at 160 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Alanyl Aminopeptidase, Membrane; Microsomal Aminopeptidase; Aminopeptidase M; Myeloid Plasma Membrane Glycoprotein CD13; Alanyl (Membrane) Aminopeptidase; Membrane Alanyl Aminopeptidase; Aminopeptidase N; EC 3.4.11.2; GP150; CD13; PEPN
- Gene ID
- 290
- UniProt ID
- P15144
- Sequence Similarities
- Belongs to the peptidase M1 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Disulfide bond, Glycoprotein, Sulfation
- Protein Refseq
- NP_001141.2; NP_001368852.1; NP_001368853.1
- Function
- Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-69-F(E) | Recombinant Anti-human ANPEP Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-69-F(E) | Recombinant Human Anti-human ANPEP Antibody Fab Fragment | WB, FC, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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