Rabbit Anti-ATM Recombinant Antibody (VS13-YC77)
CAT#: VS13-YC77
The Rabbit Anti-ATM Recombinant Antibody is specific for human ATM. ATM encodes a cell cycle checkpoint kinase critical for DNA damage response and genome stability, phosphorylating diverse proteins like p53 and BRCA1, with mutations causing ataxia telangiectasia. The anti-ATM antibody can be used for Western blot (1/500-1/5000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/1000).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong nuclear positivity in tubules and glomeruli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_5_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_4.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_2_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/102/2822_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149311-ATM
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Specifications
- Immunogen
- Recombinant human ATM, aa 1951-2000.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATM
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
- Related Disease
- Ataxia-Telangiectasia; Mantle Cell Lymphoma
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/1000
Target
- Alternative Names
- ATM Serine/Threonine Kinase; Ataxia Telangiectasia Mutated; A-T Mutated; EC 2.7.11.1; Ataxia Telangiectasia Mutated (Includes Complementation Groups A, C And D); TEL1, Telomere Maintenance 1, Homolog (S. Cerevisiae); TEL1, Telomere Maintenance 1, Homolog; Serine-Protein Kinase ATM; AT Mutated;
- Gene ID
- 472
- UniProt ID
- Q13315
- Long Name
- ATM Serine/Threonine Kinase
- Cellular Localization
- Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Nucleus; Peroxisome
- Protein Refseq
- XP_006718906.1; XP_005271619.2
- Function
- Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele.
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Related Diseases
Gastric Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ATM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0059MZ | Chicken Anti-ATM Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0286 | Hi-Affi™ Rabbit Anti-ATM Recombinant Antibody (clone DS286AB) | IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2302-CN | Rabbit Anti-ATM Recombinant Antibody (clone JM93-25) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-QX82 | Mouse Anti-ATM Recombinant Antibody (clone 7C3-3A2-5D1) | IHC | Mouse IgG1 |
| VS3-QX83 | Mouse Anti-ATM Recombinant Antibody (clone 8G1-7B2-3E1) | IHC | Mouse IgG1 |
| VS3-QX84 | Mouse Anti-ATM Recombinant Antibody (clone 9E1-10B9-1E4) | IHC | Mouse IgG1 |
| VS3-QX85 | Mouse Anti-ATM Recombinant Antibody (clone 10G6-10G10-1G8) | IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY24 | AbPlus™ Anti-ATM Magnetic Beads (4E11) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY36 | Mouse Anti-NHP ATM Recombinant Antibody (clone 2C1) | WB, IHC, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY197 | Anti-ATM Immunohistochemistry Kit | IHC | |
| VS-0525-XY599 | Anti-Mouse ATM Immunohistochemistry Kit | IHC | |
| VS-0525-XY598 | Anti-Human ATM Immunohistochemistry Kit | IHC |
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