Rabbit Anti-BCL2 Recombinant Antibody (clone 3F6) (CAT#: ZG-0104U)
Rabbit anti-BCL2 antibody (clone 3F6) displayed high specificity against the BCL2 protein of Human and Mouse.
Specific Inquiry
WB
Figure 1 Rabbit Anti-BCL2 Antibody (ZG-0104U) in WB
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U-937 whole cell lysate, THP-1 whole cell lysate, Raji whole cell lysate, Mouse Spleen whole cell lysate. All lanes: Bcl-2 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27, 23 kDa
Observed band size: 27 kDa
IHC
Figure 2 Rabbit Anti-BCL2 Antibody (ZG-0104U) in IHC
IHC image of ZG-0104U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Bcl-2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BCL2
- Species Reactivity
- Human, Mouse
- Clone
- 3F6
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Follicular Lymphoma 1 and High-Grade B-Cell Lymphoma Double-Hit/Triple-Hit.
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Bcl-2; PPP1R50; apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; protein phosphatase 1, regulatory subunit 50
- Gene ID
- 596
- UniProt ID
- P10415
Related Resources
Hedgehog Signaling Pathway
p53 Signaling Pathway
Prostate Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Amyotrophic Lateral Sclerosis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BCL2"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-103-F(E) | Recombinant Anti-dog BCL2 Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-103-F(E) | Recombinant Human Anti-dog BCL2 Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-103-S(P) | Recombinant Anti-dog BCL2 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-103-S(P) | Recombinant Human Anti-dog BCL2 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-053 | Recombinant Anti-human BCL2 VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
| HPAB-0681-YJ-VHH | Camelid Anti-BCL2 Recombinant Single Domain Antibody (CBB72YJ) | ELISA | Camelid VHH |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-041CQ | Mouse Anti-BCL2 Recombinant Antibody (clone CBL685) | Neut | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0342 | Hi-Affi™ Rabbit Anti-BCL2 Recombinant Antibody (clone DS342AB) | IHC-P | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0405 | PE-A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-CN0406 | APC-A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-CN0407 | FITC-A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-CN0408 | A*02:01/Human BCL2 (PLFDFSWLSL) MHC Tetramer | FCM, IHC | |
| MHC-CN0409 | PE-A*02:01/Human BCL2 (WLSLKTLLSL) MHC Tetramer | FCM, IHC |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1020CQ | Human Anti-BCL2 Recombinant Antibody (clone bcl-2/124) | WB, IF, FC, Apop | Human IgG1 |
| FAMAB-1021CQ | Human Anti-BCL2 Recombinant Antibody (clone bcl-2/100) | IP, WB, IHC | Human IgG1 |
| ZG-0327F | Mouse Anti-BCL2 Recombinant Antibody (ZG-0327F) | WB | Mouse IgG |
| ZG-0569J | Rabbit Anti-BCL2 Recombinant Antibody (clone 2C6) | ELISA, WB, IHC, FC | Rabbit IgG |
| VS4-WK47 | Mouse Anti-BCL2 Recombinant Antibody (clone 8E1D8) | ELISA, WB, FC | Mouse IgG1 |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0104U. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.