Rabbit Anti-BLNK Recombinant Antibody (clone R04-7H1)
CAT#: VS3-FY1666
The R04-7H1 recombinant monoclonal antibody is designed to specifically target human BLNK protein, a calculated weight of 50 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R04-7H1 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/1779_G1_1_selected.jpg
Normal Tissue
Figure 2 Tonsil
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_4_8_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/102126_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Gradient Gradient: Intensity: Not representative Quantity: None Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_7_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_7_8.jpg
Normal Tissue
Figure 7 Spleen
Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/102126_B_7_4.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (Daudi, REH, RPMI-8226, U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000095585-BLNK
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Specifications
- Immunogen
- Synthetic peptide of human BLNK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BLNK
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-7H1
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 50 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Hypoglobulinemia and absent B cells
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- B Cell Linker; B-Cell Adapter Containing A SH2 Domain Protein; Src Homology [SH2] Domain-Containing Leukocyte Protein Of 65 KD; Src Homology 2 Domain-Containing Leukocyte Protein Of 65 KDa; B-Cell Adapter Containing A Src Homology 2 Domain Protein; B Cell Adaptor Containing SH2 Domain; Cytoplasmic Adapter Protein; B-Cell Activation; SLP-65
- Gene ID
- 29760
- UniProt ID
- Q8WV28
- Cellular Localization
- Cytoplasm, Cell membrane
- Post Translation Modifications
- Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN.
Modification sites at PhosphoSitePlus (Q8WV28)
Modification sites at neXtProt (NX_Q8WV28)
- Protein Refseq
- NP_001107566.1; NP_001245369.1; NP_001245370.1; NP_001245371.1; NP_037446.1
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Related Signaling Pathways
BCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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