Recombinant Llama Anti-C3 Single Domain Antibody (PABC-558)
CAT#: PABC-558
This product is a recombinant Llama antibody that can recognize C3. hC3Nb1 against human C3b and show that it binds with lownanomolar or picomolar affinity to native C3, C3b, iC3b, and the C3(H2O) mimic C3-methylamine(C3MA) and yields efficient inhibition of the
alternative pathway in human and murine serum. hC3Nb1 is shown to prevent both proconvertase assembly as well as binding of the C3 substrate to C3 convertases. hC3Nb1 is an efficient inhibitor of FH cofactor activity.
Published Data
Gene Expression
Inhib
Figure 1 The hC3Nb1 sdAb inhibits C3 cleavage at the substrate level.
Assay in 1 % human serum on a mannan-coated LP activating surface. The horizontal axis gives the concentration of sdAbs added to the serum. The vertical axis shows the level of C3 fragment deposition with 100 % deposition defined as the signal from serum without added sdAb. The dotted line indicates the final molar concentration of C3 in the assay based on a concentration of 5.3 μM in undiluted plasma.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
Inhib
Figure 2 The hC3Nb1 is a potent inhibitor of the alternative pathway.
Assay in mouse serum on a zymosan-coated alternative pathway activating surface depicted as in panel A. The dotted line represents the final molar concentration of C3 in the assay assuming 4.2 μM C3 in 100 % serum from C57BL6 male mice.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
Inhib
Figure 3 hC3Nb1 inhibits convertase assembly and FH mediated FI cleavage of C3b due to spatial overlap with FH and FB.
SEC analysis of the AP proconvertase with and without hC3Nb1. The Nb inhibits the formation of the AP proconvertase only when the N122 linked glycan is present on FB.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
SPR
Figure 4 SPR analysis of the interaction of hC3Nb1 with C3b and C3.
Representative SPR concentration series for C3 are displayed. The measured (gray) and fitted (red) SPR curves are shown for 50, 20, 10, 5, 2, 1, 0.25 and 0.1 nM for C3b or 20, 10, 5, 2.5, 1.25, 0.61, 0.32 and 0.15 nM for C3.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
Inhib
Figure 5 The hC3Nb1 sdAb also inhibits C3 cleavage at the substrate level.
Assay in 1 % human serum on a mannan-coated LP activating surface. The horizontal axis gives the concentration of sdAbs added to the serum. The vertical axis shows the level of C3 fragment deposition with 100 % deposition defined as the signal from serum without added sdAb.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
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Normal Tissue
Figure 1 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3563/10702_A_9_5.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (BJ hTERT+, EFO-21, Hep G2, HSkMC, SiHa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125730-C3
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Specifications
- Immunogen
- Purified C3b
- Host Species
- Llama
- Type
- Llama VHH
- Specificity
- Human C3
- Species Reactivity
- Human
- Clone
- PABC-558
- Applications
- ELISA, Inhib
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay and Inhibition. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Complement Component 3; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 1; Complement Component C3a; Complement Component C3b; C3a Anaphylatoxin; Prepro-C3; CPAMD1; Epididymis Secretory Sperm Binding Protein Li 62p; Acylation-Stimulating Protein Cleavage Product; Complement Component C3; HEL-S-62p; AHUS5; ARMD9; ASP; C3a; C3b
- Gene ID
- 718
- UniProt ID
- P01024
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(Creative Biolabs Cat# PABC-558, RRID: AB_3111601)
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Related Diseases
Systemic Lupus Erythematosus
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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