Rat Anti-C3 Recombinant Antibody (clone CBLS-197)
CAT#: MOB-167CQ
Rat Antibody shows specific binding activity against Mouse C3.
Gene Expression
Normal Tissue
Figure 1 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3563/10702_A_9_5.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (BJ hTERT+, EFO-21, Hep G2, HSkMC, SiHa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125730-C3
❮
❯
Specifications
- Immunogen
- Mouse complement component 3
- Host Species
- Rat
- Type
- Rat IgG2a
- Species Reactivity
- Mouse
- Clone
- CBLS-197
- Applications
- WB, FC, IA, IF, IHC, IHC-Fr, IHC-P, IP
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- 1 mg/ml
- Buffer
- PBS with 0.05% Sodium Azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Excellent IHC on Frozen Sections
We used this rat antibody (clone CBLS-197) for immunohistochemistry on frozen sections. It provided excellent staining of mouse C3 complement protein. The morphology was well-preserved, and the signal was specific to the target tissues.
Robust for Flow Cytometry
This antibody worked very well in flow cytometry analysis of complement activation. It bound specifically to Mouse C3 and allowed us to distinguish populations clearly. It is a versatile reagent that also works in IP and Western Blot.
Q&As
-
What species reactivity does this C3 antibody show?
A: Rat Antibody (clone CBLS-197) shows specific binding activity against Mouse C3 (complement component 3).
-
What are the extensive applications for this antibody?
A: It is applicable in WB, Flow Cytometry (FC), Immunoassay (IA), Immunofluorescence (IF), IHC (Frozen and Paraffin), and Immunoprecipitation (IP).
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MOB-167CQ, RRID: AB_3713950)
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Related Diseases
Systemic Lupus Erythematosus
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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