Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.84) (CAT#: FAMAB-0252JF)
Recombinant monoclonal antibody specially binds to cardiolipin. This mouse anti-cardiolipin antibody (A1.84) provides a new sight to understand the origin and pathogenic role of autoantibodies in patients with systemic lupus erythematosus and other autoimmune disorders.
Specific Inquiry
ELISA
Figure 1 Antibodies to human βGP-I in serum in purified form detected after inhibition with cardiolipin micelles.
Error bars: +/- 1 standard error.
Cabral, A. R., Amigo, M. C., Cabiedes, J., & Alarcon-Segovia, D. (1996). The antiphospholipid/cofactor syndromes: a primary variant with antibodies to β2-glycoprotein-I but no antibodies detectable in standard antiphospholipid assays. The American journal of medicine, 101(5), 472-481.
WB
Figure 2 SDS-PAGE profile of the recombinant B3 Fab protein.
The Fab protein resolves in around 45-kDa region.
Kumar, S., Kalsi, J., Ravirajan, C. T., Rahman, A., Athwal, D., Latchman, D. S., ... & Pearl, L. H. (2000). Molecular Cloning and Expression of the Fabs of Human Autoantibodies in Escherichia coli DETERMINATION OF THE HEAVY OR LIGHT CHAIN CONTRIBUTION TO THE ANTI-DNA/-CARDIOLIPIN ACTIVITY OF THE Fab. Journal of Biological Chemistry, 275(45), 35129-35136.
IHC
Figure 3 Binding of the purified recombinant B3 Fab protein to DNA in situ in Crithidia assay.
Original magnification ×50.
Kumar, S., Kalsi, J., Ravirajan, C. T., Rahman, A., Athwal, D., Latchman, D. S., ... & Pearl, L. H. (2000). Molecular Cloning and Expression of the Fabs of Human Autoantibodies in Escherichia coli DETERMINATION OF THE HEAVY OR LIGHT CHAIN CONTRIBUTION TO THE ANTI-DNA/-CARDIOLIPIN ACTIVITY OF THE Fab. Journal of Biological Chemistry, 275(45), 35129-35136.
Specifications
- Host Species
- Mouse
- Derivation
- Mouse phage display scFv library
- Type
- Mouse IgG
- Specificity
- Cardiolipin
- Clone
- A1.84
- Applications
- WB, ELISA, IHC
- Related Disease
- Systemic lupus erythematosus, other autoimmune disorders
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Cardiolipin antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
Target
- Alternative Names
- 1,3-bis(sn-3'-phosphatidyl)-sn-glycerol; Cardiolipin
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone A1.84"
See other products for "Cardiolipin"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0251JF | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.72) | WB, ELISA, IHC | Mouse IgG |
| FAMAB-0458WJ | Human Anti-Cardiolipin Recombinant Antibody (clone RSP4) | ELISA, WB | Human IgM, λ |
| FAMAB-0459WJ | Human Anti-Cardiolipin Recombinant Antibody (clone IS4) | ELISA, WB | Human IgG |
| FAMAB-0460WJ | Human Anti-Cardiolipin Recombinant Antibody (clone B3) | ELISA, WB | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0251JF-F(E) | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.72); Fab Fragment | IB, ELISA, FuncS | Mouse Fab |
| FAMAB-0252JF-F(E) | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.84); Fab Fragment | IB, ELISA, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0251JF-S(P) | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.72); scFv Fragment | IB, ELISA, FuncS | Mouse scFv |
| FAMAB-0252JF-S(P) | Mouse Anti-Cardiolipin Recombinant Antibody (clone A1.84); scFv Fragment | IB, ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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