Human Anti-CCL5 Recombinant Antibody (clone 1D9) (CAT#: HPAB-0380LY)

This product is an antibody that recognizes RANTES. The antibody 1D9 can be used for immunoassay techniques such as: ELISA, Inhibition.


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FC

Figure 1 The antibody 1D9 is specific for CCR2.

Figure 1 The antibody 1D9 is specific for CCR2.

L1.2 stable transfectants bearing either CCR2b, CCR5, or CCR1 on their cell surface were analyzed by flow cytometry following incubation of the cells with the specific primary antibodies 1D9 (anti-CCR2), 2D7 (anti-CCR5), or 2D4 (anti-CCR1) all at 10 g/ml and subsequent detection with a goat anti-mouse fluorescein isothiocyanate-conjugated secondary mAb.

Martinelli, R., Sabroe, I., LaRosa, G., Williams, T. J., & Pease, J. E. (2001). The CC chemokine eotaxin (CCL11) is a partial agonist of CC chemokine receptor 2b. Journal of Biological Chemistry, 276(46), 42957-42964.

IF

Figure 2 Effects of irradiations on melanoma blood vessels.

Figure 2 Effects of irradiations on melanoma blood vessels.

Illustration of the methodology. White contours: CD31+. Green staining: FITC-albumin+. Arrows: nonperfused vessels (CD31+ and FITC-albumin–).

Potez, M., Fernandez-Palomo, C., Bouchet, A., Trappetti, V., Donzelli, M., Krisch, M., ... & Djonov, V. (2019). Synchrotron Microbeam Radiation Therapy as a New Approach for the Treatment of Radioresistant Melanoma: Potential Underlying Mechanisms. International Journal of Radiation Oncology* Biology* Physics, 105(5), 1126-1136.

IF

Figure 3 Effects of irradiations on melanoma tumor cells turnover.

Figure 3 Effects of irradiations on melanoma tumor cells turnover.

Representative images of microbeam radiation therapy (MRT) (left column) and broad beam (BB) (right column) irradiated tumors immunostained by KI67 (red) and DAPI (blue) at D5 and D9.


Specifications

  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • Human IgG1
  • Specificity
  • Human CCL5
  • Species Reactivity
  • Human
  • Clone
  • 1D9
  • Applications
  • ELISA, Inhib, FC, IF

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The RANTES antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition, Flow Cytometry, Immunofluorescence.

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 1D9"

See other products for "CCL5"

Human Antibody

CAT Product Name Application Type
TAB-098CL Human Anti-CCL5 Recombinant Antibody (TAB-098CL) ELISA Human IgG

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0487MZ Chicken Anti-RANTES Polyclonal IgY Indirect ELISA, WB Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-459CQ Rat Anti-CCL5 Recombinant Antibody (clone 53405.111) IF, Inhib Rat IgG2a

Rabbit Monoclonal Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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