Rabbit Anti-CCND1 Recombinant Antibody (clone 5D8)
CAT#: ZG-0118U
Rabbit anti-CCND1 antibody (clone 5D8) displayed high specificity against the CCND1 protein of Human and Mouse.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-CCND1 Antibody (ZG-0118U) in WB
Positive WB detected in: NIH/3T3 whole cell lysate, SH-SY5Y whole cell lysate, U-87 whole cell lysate. All lanes: Cyclin D1 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
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Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1667_B12_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in a fraction of basal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1607_B12_2_red_green.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_9_4.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (U-266/70, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110092-CCND1
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Specifications
- Immunogen
- A synthesized peptide derived from human Cyclin D1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CCND1
- Species Reactivity
- Human, Mouse
- Clone
- 5D8
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Von Hippel-Lindau Syndrome and Myeloma, Multiple.
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
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Related Diseases
Breast Cancer
Related Signaling Pathways
Hedgehog Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY148 | Mouse Anti-NHP CCND1 Recombinant Antibody (clone DCS-6) | WB, IF | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1085 | Anti-CCND1 Immunohistochemistry Kit | IHC | |
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