Mouse Anti-NHP CCND1 Recombinant Antibody (clone DCS-6)
CAT#: VS-1024-XY148
The mouse-derived antibody is finely tuned to recognize CCND1 across a diverse array of species, including dog, human, mouse, non-human primate, and rat. With an immunogen of full-length recombinant human cyclin D1 protein, it offers exceptional specificity. Suitable for Western blot (WB) and immunofluorescence (IF) applications, this antibody ensures accurate detection and analysis of CCND1, making it an essential tool for in-depth studies of cell cycle dynamics and related research fields.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1667_B12_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in a fraction of basal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1607_B12_2_red_green.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_9_4.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (U-266/70, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110092-CCND1
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Specifications
- Immunogen
- Human recombinant full length cyclin D1 protein
- Host Species
- Mouse
- Type
- Mouse IgG2a, kappa
- Specificity
- CCND1
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- DCS-6
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.2% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:800, IF: 1-2 µg/mL
Target
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Related Diseases
Breast Cancer
Related Signaling Pathways
Hedgehog Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone DCS-6"
- CAT
- Product Name
See other products for "CCND1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G004R | Anti-Ccnd1 immunotoxin (IgG)-RTA | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0110CT | Mouse Anti-CCND1 Recombinant Antibody (clone 4E9) | ELISA, WB | Mouse IgG1 |
| MRO-1799-CN | Rabbit Anti-CCND1 Polyclonal Antibody (MRO-1799-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1800-CN | Rabbit Anti-CCND1 Polyclonal Antibody (MRO-1800-CN) | WB, IHC | Rabbit IgG |
| ZG-0446F | Mouse Anti-CCND1 Recombinant Antibody (ZG-0446F) | WB, ELISA | Mouse IgG |
| ZG-0424U | Rabbit Anti-CCND1 Recombinant Antibody (clone E20-D), FITC | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-262CQ | Mouse Anti-CCND1 Recombinant Antibody (clone DCS-6) | IHC-Fr, WB, Block, IHC-P, IF, FC, IP | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0515 | Hi-Affi™ Rabbit Anti-CCND1 Recombinant Antibody (clone DS515AB) | IHC | Rabbit IgG |
| MOR-4566 | Hi-Affi™ Rabbit Anti-CCND1 Recombinant Antibody (clone TH76DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1085 | Anti-CCND1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1086 | Anti-Mouse CCND1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1087 | Anti-Monkey CCND1 Immunohistochemistry Kit | IHC |
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