Rabbit Anti-CCNE1 Recombinant Antibody (VS13-YC149)
CAT#: VS13-YC149
The Rabbit Anti-CCNE1 Recombinant Antibody is specific for human and mouse CCNE1. CCNE1 encodes cyclin E1, vital for cell cycle regulation during G1/S transition, with overexpression linked to tumors, influencing chromosome stability and cell cycle progression, including NPAT phosphorylation. The anti-CCNE1 antibody can be used for Western blot (1/500-1/1000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/if_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_4_6.jpg
Normal Tissue
Figure 3 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/308/2153_A_1_7.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16682/36096_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105173-CCNE1
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Specifications
- Immunogen
- Recombinant human Cyclin E1, aa 361-410.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CCNE1
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
- Related Disease
- Tumor
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- Cyclin E1; CCNE; G1/S-Specific Cyclin-E1; Cyclin Es; Cyclin Et; PCCNE1;
- Gene ID
- 898
- UniProt ID
- P24864
- Long Name
- Cyclin E1
- Sequence Similarities
- Belongs to the cyclin family. Cyclin E subfamily.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001229.1; NP_001309191.1
- Function
- Essential for the control of the cell cycle at the G1/S (start) transition.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CCNE1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B078(A) | Recombinant Anti-human CCNE1 Intrabody [(D-Arg)9] | FACS, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B078(G) | Recombinant Anti-human CCNE1 Intrabody [+36 GFP] | PCA, WB, FuncS | scFv-Tat |
| IAB-B078(T) | Recombinant Anti-human CCNE1 Intrabody [Tat] | FACS, ELISA, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0112CT | Mouse Anti-CCNE1 Recombinant Antibody (clone 0.U.307) | ELISA | Mouse IgG |
| MRO-0415-CN | Rabbit Anti-CCNE1 Recombinant Antibody (clone CBACN-166) | WB, IF | Rabbit IgG |
| ZG-0683F | Mouse Anti-CCNE1 Recombinant Antibody (clone 168) | WB, IP, WB | Mouse IgG2a |
| VS3-CJ810 | Rabbit Anti-CCNE1 Recombinant Antibody (VS3-CJ810) | WB, ICC, IF | Rabbit IgG |
| VS3-CJ811 | Rabbit Anti-CCNE1 (phospho T77) Recombinant Antibody (VS3-CJ811) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0517 | Hi-Affi™ Rabbit Anti-CCNE1 Recombinant Antibody (clone DS517AB) | FC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1090 | Anti-CCNE1 Immunohistochemistry Kit | IHC |
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